Literature DB >> 20400862

Development and characterization of monoclonal antibodies against Rift Valley fever virus nucleocapsid protein generated by DNA immunization.

Raquel Martín-Folgar1, Gema Lorenzo, Hani Boshra, Javier Iglesias, Francisco Mateos, Belén Borrego, Alejandro Brun.   

Abstract

This paper describes the generation of monoclonal antibodies directed to immunogenic nucleoprotein N epitopes of Rift Valley fever virus (RVFV), and their application in diagnostics, both for antibody detection in competitive ELISA and for antigen capture in a sandwich ELISA. Monoclonal antibodies (mAbs) were generated after DNA immunization of Balb/c mice and characterized by western blot, ELISA and cell immunostaining assays. At least three different immunorelevant epitopes were defined by mAb competition assays. Interestingly, two of the mAbs generated were able to distinguish between RVFV strains from Egyptian or South African lineages. These monoclonal antibodies constitute useful tools for diagnosis, especially for the detection of serum anti-RVFV antibodies from a broad range of species by means of competitive ELISA.

Entities:  

Mesh：

Substances：

Year:  2010        PMID： 20400862      PMCID： PMC2881254          DOI： 10.4161/mabs.2.3.11676

Source DB:  PubMed          Journal:  MAbs        ISSN： 1942-0862            Impact factor:   5.857


  28 in total

1.  [Climate change in spain and risk of infectious and parasitic diseases transmitted by arthropods and rodents].

Authors:  Rogelio López-Vélez; Ricardo Molina Moreno
Journal:  Rev Esp Salud Publica       Date:  2005 Mar-Apr

2.  Topological mapping of antigenic sites on the Rift Valley fever virus envelope glycoproteins using monoclonal antibodies.

Authors:  T G Besselaar; N K Blackburn
Journal:  Arch Virol       Date:  1991       Impact factor: 2.574

3.  The synergistic neutralization of Rift Valley fever virus by monoclonal antibodies to the envelope glycoproteins.

Authors:  T G Besselaar; N K Blackburn
Journal:  Arch Virol       Date:  1992       Impact factor: 2.574

4.  A study of the effect of chemical inactivants on the epitopes of Rift Valley fever virus glycoproteins using monoclonal antibodies.

Authors:  N K Blackburn; T G Besselaar
Journal:  J Virol Methods       Date:  1991-08       Impact factor: 2.014

5.  The N terminus of Rift Valley fever virus nucleoprotein is essential for dimerization.

Authors:  Nicolas Le May; Nicolas Gauliard; Agnès Billecocq; Michèle Bouloy
Journal:  J Virol       Date:  2005-09       Impact factor: 5.103

6.  An inhibition enzyme-linked immunosorbent assay for the detection of antibody to Rift Valley fever virus in humans, domestic and wild ruminants.

Authors:  Janusz T Paweska; Elizabeth Mortimer; Patricia A Leman; Robert Swanepoel
Journal:  J Virol Methods       Date:  2005-04-11       Impact factor: 2.014

Review 7.  Rift Valley fever.

Authors:  G H Gerdes
Journal:  Rev Sci Tech       Date:  2004-08       Impact factor: 1.181

8.  Production of monoclonal antibodies against Rift Valley fever virus Application for rapid diagnosis tests (virus detection and ELISA) in human sera.

Authors:  A Zaki; D Coudrier; A I Yousef; M Fakeeh; M Bouloy; A Billecocq
Journal:  J Virol Methods       Date:  2005-08-15       Impact factor: 2.014

9.  The effect of neutralizing monoclonal antibodies on early events in Rift Valley fever virus infectivity.

Authors:  T G Besselaar; N K Blackburn
Journal:  Res Virol       Date:  1994 Jan-Feb

10.  Recurrence of Rift Valley fever in Egypt.

Authors:  R R Arthur; M S el-Sharkawy; S E Cope; B A Botros; S Oun; J C Morrill; R E Shope; R G Hibbs; M A Darwish; I Z Imam
Journal:  Lancet       Date:  1993-11-06       Impact factor: 79.321
View more
  20 in total

1.  Antigenicity of severe fever with thrombocytopenia syndrome virus nucleocapsid protein and its potential application in the virus serodiagnosis.

Authors:  Abulimiti Moming; Yujiang Zhang; Chenchen Chang; Huan Yu; Meifang Wang; Zhihong Hu; Fei Deng; Surong Sun
Journal:  Virol Sin       Date:  2017-02       Impact factor: 4.327

2.  Reagents for detection of Rift Valley fever virus infection in sheep.

Authors:  Brian J Shiell; Siying Ye; Jennifer A Harper; Brenda van der Heide; Gary Beddome; Adam J Foord; Wojtek P Michalski; John Bingham; Grantley R Peck
Journal:  J Vet Diagn Invest       Date:  2020-05-25       Impact factor: 1.279

3.  Potent monoclonal antibodies against Clostridium difficile toxin A elicited by DNA immunization.

Authors:  Chunhua Zhang; Ke Jin; Yanling Xiao; Ying Cheng; Zuhu Huang; Shixia Wang; Shan Lu
Journal:  Hum Vaccin Immunother       Date:  2013-07-12       Impact factor: 3.452

4.  Creation of Rift Valley fever viruses with four-segmented genomes reveals flexibility in bunyavirus genome packaging.

Authors:  Paul J Wichgers Schreur; Nadia Oreshkova; Rob J M Moormann; Jeroen Kortekaas
Journal:  J Virol       Date:  2014-07-09       Impact factor: 5.103

5.  Development and Characterization of Monoclonal Antibodies Directed Against the Nucleoprotein of Heartland Virus.

Authors:  Amanda E Calvert; Aaron C Brault
Journal:  Am J Trop Med Hyg       Date:  2015-10-26       Impact factor: 2.345

6.  Lithium inhibits NF-κB nuclear translocation and modulate inflammation profiles in Rift valley fever virus-infected Raw 264.7 macrophages.

Authors:  Raymond Tshepiso Makola; Joe Kgaladi; Garland Kgosi More; Petrus Jansen van Vuren; Janusz Tadeusz Paweska; Thabe Moses Matsebatlela
Journal:  Virol J       Date:  2021-06-04       Impact factor: 4.099

7.  Critical epitopes in the nucleocapsid protein of SFTS virus recognized by a panel of SFTS patients derived human monoclonal antibodies.

Authors:  Li Yu; Li Zhang; Lina Sun; Jing Lu; Wei Wu; Chuan Li; Quanfu Zhang; Fushun Zhang; Cong Jin; Xianjun Wang; Zhenqiang Bi; Dexin Li; Mifang Liang
Journal:  PLoS One       Date:  2012-06-12       Impact factor: 3.240

8.  Rift Valley and West Nile virus antibodies in camels, North Africa.

Authors:  Mehdi El-Harrak; Raquel Martín-Folgar; Francisco Llorente; Paloma Fernández-Pacheco; Alejandro Brun; Jordi Figuerola; Miguel Angel Jiménez-Clavero
Journal:  Emerg Infect Dis       Date:  2011-12       Impact factor: 6.883

9.  The nucleocapsid protein of Rift Valley fever virus is a potent human CD8+ T cell antigen and elicits memory responses.

Authors:  Weidong Xu; Douglas M Watts; Margaret C Costanzo; Xiaolei Tang; Leon A Venegas; Feng Jiao; Alessandro Sette; John Sidney; Andrew K Sewell; Linda Wooldridge; Shinji Makino; John C Morrill; Clarence J Peters; June Kan-Mitchell
Journal:  PLoS One       Date:  2013-03-18       Impact factor: 3.240

10.  A single immunization with MVA expressing GnGc glycoproteins promotes epitope-specific CD8+-T cell activation and protects immune-competent mice against a lethal RVFV infection.

Authors:  Elena López-Gil; Gema Lorenzo; Esther Hevia; Belén Borrego; Martin Eiden; Martin Groschup; Sarah C Gilbert; Alejandro Brun
Journal:  PLoS Negl Trop Dis       Date:  2013-07-11
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.