OBJECTIVE: To explore oocyte competence for subsequent birth. The modified natural IVF/intracytoplasmic sperm injection (ICSI) cycle was used as an experimental model by measuring levels of cytokines, chemokines, and growth factors in individual follicular fluids (FF). DESIGN: A retrospective blinded study. SETTING: European network of research, Embryo Implantation Control (EMBIC). PATIENT(S): Single FF from 83 women were analyzed during a modified natural IVF/ICSI cycle, and reproducibility of follicular composition was evaluated over two cycles for 15 patients. INTERVENTION(S): Each FF sample was blindly tested to assess levels of 26 factors by bead-based immunoassays. MAIN OUTCOME MEASURE(S): Each mediator was evaluated as a potential biomarker of subsequent birth by multivariate regression analysis. RESULT(S): A combination of both FF G-CSF and IL-15 was the optimal model to predict birth (AUC(ROC), 0.85). Birth rates per cycle were 48.9% (16/33) if two good-prognosis criteria were present (FF G-CSF>12 pg/mL and IL-15<7 pg/mL) and 8% (3/36) and 0% (0/14) if, respectively, one or none were present. FF G-CSF was significantly correlated over two cycles (r=.71), suggesting a possible prognostic value of its documentation. CONCLUSION(S): Combined follicular G-CSF and IL-15 quantification appears as an efficient and noninvasive method to define oocyte competence for subsequent successful conception in modified natural IVF/ICSI cycles.
OBJECTIVE: To explore oocyte competence for subsequent birth. The modified natural IVF/intracytoplasmic sperm injection (ICSI) cycle was used as an experimental model by measuring levels of cytokines, chemokines, and growth factors in individual follicular fluids (FF). DESIGN: A retrospective blinded study. SETTING: European network of research, Embryo Implantation Control (EMBIC). PATIENT(S): Single FF from 83 women were analyzed during a modified natural IVF/ICSI cycle, and reproducibility of follicular composition was evaluated over two cycles for 15 patients. INTERVENTION(S): Each FF sample was blindly tested to assess levels of 26 factors by bead-based immunoassays. MAIN OUTCOME MEASURE(S): Each mediator was evaluated as a potential biomarker of subsequent birth by multivariate regression analysis. RESULT(S): A combination of both FF G-CSF and IL-15 was the optimal model to predict birth (AUC(ROC), 0.85). Birth rates per cycle were 48.9% (16/33) if two good-prognosis criteria were present (FF G-CSF>12 pg/mL and IL-15<7 pg/mL) and 8% (3/36) and 0% (0/14) if, respectively, one or none were present. FF G-CSF was significantly correlated over two cycles (r=.71), suggesting a possible prognostic value of its documentation. CONCLUSION(S): Combined follicular G-CSF and IL-15 quantification appears as an efficient and noninvasive method to define oocyte competence for subsequent successful conception in modified natural IVF/ICSI cycles.
Authors: S Spanou; D Kalogiannis; E Zapanti; M Gazouli; I A Sfontouris; C Siristatidis; George Mastorakos Journal: J Assist Reprod Genet Date: 2018-04-04 Impact factor: 3.412
Authors: N Lédée; V Gridelet; S Ravet; C Jouan; O Gaspard; F Wenders; F Thonon; N Hincourt; M Dubois; J M Foidart; C Munaut; S Perrier d'Hauterive Journal: Hum Reprod Date: 2012-12-06 Impact factor: 6.918
Authors: Jennifer H Southcombe; Nathalie Lédée; Sophie Perrier d'Hauterive; Karen Turner; Tim Child; James V Snider; Christopher W G Redman; Ian L Sargent; Ingrid Granne Journal: PLoS One Date: 2013-09-10 Impact factor: 3.240