Characterisation and differential regulation of MAFbx/Atrogin-1 alpha and beta transcripts in skeletal muscle of Atlantic salmon (Salmo salar).

MAFbx is an E3 ubiquitin ligase which plays important roles in myogenesis and muscle atrophy. We characterised the Atlantic salmon MAFbx gene, identifying two alternatively spliced MAFbx isoforms. The mRNA sequence of Atlantic salmon MAFbx-alpha is 1698 nucleotides long including a 134 bp 5' UTR and 1065 bp coding sequence which encodes a 355 amino acid protein with a predicted mass of 41,657Da and pI 8.74. Two different 3' UTRs were identified of 495 and 314 bp in length. MAFbx-beta is produced by the removal of the 116 bp exon 2 from MAFbx-alpha, resulting in a frame shift mutation and introduction of a premature stop codon. In contrast to mammals, MAFbx-alpha and beta were ubiquitously expressed in all salmon tissues examined. In vivo, expression was 600-fold (MAFbx-alpha) and 200-fold (MAFbx-beta) higher in fasted individuals than following 21 days refeeding to satiation. In primary myogenic cell cultures, MAFbx-alpha mRNA was highest in differentiated myotubes while MAFbx-beta mRNA had peak expression in mono-nucleated cells. Starving cells of serum and amino acids resulted in a 6-fold increase in MAFbx-alpha, whereas MAFbx-beta remained similar to control levels. In starved cells, MAFbx-alpha mRNA levels declined in response to amino acid, IGF-I and IGF-II treatments whereas MAFbx-beta levels only decreased in response to IGF-I. Addition of amino acids and IGF or insulin to starved cells increased MAFbx-beta levels after 12 and 24h. These results indicate that regulation of MAFbx in Atlantic salmon occurs at both the transcriptional and post-transcriptional level through production of the alternatively spliced MAFbx-beta, which is a likely target for non-sense mediated decay. Copyright (c) 2010 Elsevier Inc. All rights reserved.