Literature DB >> 2039513

Southern hybridization analysis of the mecA deletion from methicillin-resistant Staphylococcus aureus.

A Wada1, Y Katayama, K Hiramatsu, T Yokota.   

Abstract

Genomic organization of methicillin-resistant Staphylococcus aureus strains and their methicillin-susceptible subclones were analyzed by pulsed-field gel electrophoresis and Southern hybridization with DNA fragments of methicillin-resistance gene mecA and an insertion element IS431 as probes. The entire mecA gene was deleted in all the seven methicillin-susceptible subclones studied, and the size of the deletion varied from 20 to 100 kilobases depending on each subclone. In six of the seven subclones, however, the downstream deletion end points were confined within a 2.0 kilobase HindIII-HindIII fragment containing a part of IS431 which was located 2.6 kilobase downstream of mecA gene. The results indicated that the intramolecular transposition of IS431 is responsible for the mecA deletion in methicillin-resistant Staphylococcus aureus.

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Year:  1991        PMID： 2039513     DOI： 10.1016/0006-291x(91)90430-f

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  27 in total

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2.  The Staphylococcus aureus mec determinant comprises an unusual cluster of direct repeats and codes for a gene product similar to the Escherichia coli sn-glycerophosphoryl diester phosphodiesterase.

Authors:  C Ryffel; R Bucher; F H Kayser; B Berger-Bächi
Journal:  J Bacteriol       Date:  1991-12       Impact factor: 3.490

3.  Staphylococcal cassette chromosome mec (SCCmec) typing of methicillin-resistant Staphylococcus aureus strains isolated in 11 Asian countries: a proposal for a new nomenclature for SCCmec elements.

Authors:  Piriyaporn Chongtrakool; Teruyo Ito; Xiao Xue Ma; Yoko Kondo; Suwanna Trakulsomboon; Chuntima Tiensasitorn; Mantana Jamklang; Tavinun Chavalit; Jae-Hoon Song; Keiichi Hiramatsu
Journal:  Antimicrob Agents Chemother       Date:  2006-03       Impact factor: 5.191

4.  Assessment of resolution and intercenter reproducibility of results of genotyping Staphylococcus aureus by pulsed-field gel electrophoresis of SmaI macrorestriction fragments: a multicenter study.

Authors:  A van Belkum; W van Leeuwen; M E Kaufmann; B Cookson; F Forey; J Etienne; R Goering; F Tenover; C Steward; F O'Brien; W Grubb; P Tassios; N Legakis; A Morvan; N El Solh; R de Ryck; M Struelens; S Salmenlinna; J Vuopio-Varkila; M Kooistra; A Talens; W Witte; H Verbrugh
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5.  Mutational analyses of open reading frames within the vraSR operon and their roles in the cell wall stress response of Staphylococcus aureus.

Authors:  N McCallum; P Stutzmann Meier; R Heusser; B Berger-Bächi
Journal:  Antimicrob Agents Chemother       Date:  2011-01-10       Impact factor: 5.191

6.  First report of methicillin-resistant Staphylococcus aureus with reduced susceptibility to vancomycin in Thailand.

Authors:  S Trakulsomboon; S Danchaivijitr; Y Rongrungruang; C Dhiraputra; W Susaemgrat; T Ito; K Hiramatsu
Journal:  J Clin Microbiol       Date:  2001-02       Impact factor: 5.948

7.  In vivo survival of teicoplanin-resistant Staphylococcus aureus and fitness cost of teicoplanin resistance.

Authors:  N McCallum; H Karauzum; R Getzmann; M Bischoff; P Majcherczyk; B Berger-Bächi; R Landmann
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8.  Molecular cloning and mapping of 16S-23S rRNA gene complexes of Staphylococcus aureus.

Authors:  A Wada; H Ohta; K Kulthanan; K Hiramatsu
Journal:  J Bacteriol       Date:  1993-11       Impact factor: 3.490

9.  Detection of the Staphylococcal mecA gene by chemiluminescent DNA hybridization.

Authors:  C P Kolbert; J E Connolly; M J Lee; D H Persing
Journal:  J Clin Microbiol       Date:  1995-08       Impact factor: 5.948

10.  New real-time PCR assay for rapid detection of methicillin-resistant Staphylococcus aureus directly from specimens containing a mixture of staphylococci.

Authors:  A Huletsky; R Giroux; V Rossbach; M Gagnon; M Vaillancourt; M Bernier; F Gagnon; K Truchon; M Bastien; F J Picard; A van Belkum; M Ouellette; P H Roy; M G Bergeron
Journal:  J Clin Microbiol       Date:  2004-05       Impact factor: 5.948
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