Literature DB >> 2039502

A novel spermidine-dependent endoribonuclease activity caused by RNA-protein complex in mouse FM3A cell extracts.

M Nashimoto1, R Kominami, S Nishi, Y Mishima.   

Abstract

We have found a novel spermidine-dependent endoribonuclease activity in mouse FM3A cell extracts. This endoribonuclease cleaves RNA substrates containing a sequence CCCCCGGUUUGU in its middle. This activity is lost either by heat- or micrococcal nuclease-pretreatment. When heat-pretreated extracts and micrococcal nuclease-pretreated ones are mixed, the activity is restored, suggesting that this activity requires both RNA and protein components. Testing the restoration of the lost endoribonuclease activity in micrococcal nuclease-pretreated extracts by addition of fractionated cellular RNAs, we identified an approximately 65 nucleotide RNA required for this endoribonuclease activity.

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Year:  1991        PMID： 2039502     DOI： 10.1016/0006-291x(91)90407-x

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  6 in total

1.  A novel 4-base-recognizing RNA cutter that can remove the single 3' terminal nucleotides from RNA molecules.

Authors:  Hiroaki Takaku; Asako Minagawa; Masamichi Takagi; Masayuki Nashimoto
Journal:  Nucleic Acids Res       Date:  2004-06-28       Impact factor: 16.971

2.  Characterization of the spermidine-dependent, sequence-specific endoribonuclease that requires transfer RNA for its activity.

Authors:  M Nashimoto
Journal:  Nucleic Acids Res       Date:  1992-07-25       Impact factor: 16.971

3.  Conversion of mammalian tRNA 3' processing endoribonuclease to four-base-recognizing RNA cutters.

Authors:  M Nashimoto
Journal:  Nucleic Acids Res       Date:  1995-09-25       Impact factor: 16.971

4.  3' truncated tRNAArg is essential for in vitro specific cleavage of partially synthesized mouse 18S rRNA.

Authors:  M Nashimoto
Journal:  Nucleic Acids Res       Date:  1993-10-11       Impact factor: 16.971

5.  A candidate prostate cancer susceptibility gene encodes tRNA 3' processing endoribonuclease.

Authors:  Hiroaki Takaku; Asako Minagawa; Masamichi Takagi; Masayuki Nashimoto
Journal:  Nucleic Acids Res       Date:  2003-05-01       Impact factor: 16.971

Review 6.  TRUE Gene Silencing.

Authors:  Masayuki Nashimoto
Journal:  Int J Mol Sci       Date:  2022-05-11       Impact factor: 6.208
  6 in total

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