| Literature DB >> 2039457 |
K R Rogers1, C J Morris, D R Blake.
Abstract
Sublethal doses of H2O2, which induces oxidative stress, cause substantial alteration to the vimentin cytoskeleton in various cell types. We have used a thiol-blot assay to assess thiol status in individual proteins from cell extracts. Vimentin thiol is oxidized in preference to other cytoskeleton proteins. Immunoblot analysis also demonstrated a loss of reactivity to an anti-vimentin monoclonal antibody under non-reducing conditions, possibly due to thiol-group oxidation. During induced oxidative stress a number of proteins become associated with the cytoskeleton extracts.Entities:
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Year: 1991 PMID: 2039457 PMCID: PMC1150123 DOI: 10.1042/bj2750789
Source DB: PubMed Journal: Biochem J ISSN: 0264-6021 Impact factor: 3.857