Literature DB >> 20392923

Rapid diagnosis of Old World Leishmaniasis by high-resolution melting analysis of the 7SL RNA gene.

Abedelmajeed Nasereddin1, Charles L Jaffe.   

Abstract

High-resolution melt analysis PCR (HRM PCR) for diagnosis of Old World Leishmania was developed using the 7SL RNA gene. Cutaneous leishmaniasis samples were analyzed. Sensitivity and specificity of HRM PCR were significantly better (P < 0.001) than those of internal transcribed spacer 1 PCR and similar to those of kinetoplast DNA PCR.

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Year:  2010        PMID： 20392923      PMCID： PMC2884505          DOI： 10.1128/JCM.00553-10

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


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