Literature DB >> 20392909

Detection of a mixed infection in a culture-negative brain abscess by broad-spectrum bacterial 16S rRNA gene PCR.

Peter M Keller1, Silvana K Rampini, Guido V Bloemberg.   

Abstract

We describe the identification of two bacterial pathogens from a culture-negative brain abscess by the use of broad-spectrum 16S rRNA gene PCR. Simultaneous detection of Fusobacterium nucleatum and Porphyromonas endodontalis was possible due to a 24-bp length difference of their partially amplified 16S rRNA genes, which allowed separation by high-resolution polyacrylamide gel electrophoresis.

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Year:  2010        PMID： 20392909      PMCID： PMC2884506          DOI： 10.1128/JCM.01922-09

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  9 in total

1.  Direct detection of bacterial pathogens in brain abscesses by polymerase chain reaction amplification and sequencing of partial 16S ribosomal deoxyribonucleic acid fragments.

Authors:  Jui-Chang Tsai; Lee-Jene Teng; Po-Ren Hsueh
Journal:  Neurosurgery       Date:  2004-11       Impact factor: 4.654

Review 2.  The role of anaerobic bacteria in sinusitis.

Authors:  Itzhak Brook
Journal:  Anaerobe       Date:  2005-10-12       Impact factor: 3.331

3.  Comparison of the microbiota of supra- and subgingival plaque in health and periodontitis.

Authors:  L A Ximénez-Fyvie; A D Haffajee; S S Socransky
Journal:  J Clin Periodontol       Date:  2000-09       Impact factor: 8.728

4.  Molecular diagnosis of bacterial endocarditis by broad-range PCR amplification and direct sequencing.

Authors:  D Goldenberger; A Künzli; P Vogt; R Zbinden; M Altwegg
Journal:  J Clin Microbiol       Date:  1997-11       Impact factor: 5.948

Review 5.  Then and now: use of 16S rDNA gene sequencing for bacterial identification and discovery of novel bacteria in clinical microbiology laboratories.

Authors:  P C Y Woo; S K P Lau; J L L Teng; H Tse; K-Y Yuen
Journal:  Clin Microbiol Infect       Date:  2008-10       Impact factor: 8.067

6.  Etiologic diagnosis of infective endocarditis by broad-range polymerase chain reaction: a 3-year experience.

Authors:  Philipp Peter Bosshard; Andreas Kronenberg; Reinhard Zbinden; Christian Ruef; Erik Christian Böttger; Martin Altwegg
Journal:  Clin Infect Dis       Date:  2003-07-09       Impact factor: 9.079

7.  Aetiological diagnosis of brain abscesses and spinal infections: application of broad range bacterial polymerase chain reaction analysis.

Authors:  L Kupila; K Rantakokko-Jalava; J Jalava; S Nikkari; R Peltonen; O Meurman; R J Marttila; E Kotilainen; P Kotilainen
Journal:  J Neurol Neurosurg Psychiatry       Date:  2003-06       Impact factor: 10.154

8.  Analysis of 525 samples to determine the usefulness of PCR amplification and sequencing of the 16S rRNA gene for diagnosis of bone and joint infections.

Authors:  Florence Fenollar; Véronique Roux; Andréas Stein; Michel Drancourt; Didier Raoult
Journal:  J Clin Microbiol       Date:  2006-03       Impact factor: 5.948

9.  The expansion of the microbiological spectrum of brain abscesses with use of multiple 16S ribosomal DNA sequencing.

Authors:  Mouhamad Al Masalma; Fabrice Armougom; W Michael Scheld; Henri Dufour; Pierre-Hugues Roche; Michel Drancourt; Didier Raoult
Journal:  Clin Infect Dis       Date:  2009-05-01       Impact factor: 9.079
  9 in total
  4 in total

1.  Fusobacterium nucleatum prosthetic hip infection in an adult with sickle cell-beta thalassemia.

Authors:  K Verma; P C McNabb; W Kurtz; J Green; C H Trabue
Journal:  Infection       Date:  2011-10-15       Impact factor: 3.553

2.  Oral flora meningoencephalitis diagnosis by next-generation DNA sequencing.

Authors:  Eric Heintz; Matthew A Pettengill; Mohamed Azhar Gangat; Dwight J Hardy; William Bonnez; Mohammed Mahdee Sobhanie
Journal:  Access Microbiol       Date:  2019-08-19

3.  Microbiological spectrum of brain abscess at a tertiary care hospital in South India: 24-year data and review.

Authors:  V Lakshmi; P Umabala; K Anuradha; K Padmaja; C Padmasree; A Rajesh; A K Purohit
Journal:  Patholog Res Int       Date:  2011-11-16

4.  Rapid brain death caused by a cerebellar abscess with Fusobacterium nucleatum in a young man with drug abuse: a case report.

Authors:  Gunnar T R Hischebeth; Vera C Keil; Katrin Gentil; Azize Boström; Klaus Kuchelmeister; Isabelle Bekeredjian-Ding
Journal:  BMC Res Notes       Date:  2014-06-10
  4 in total

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