OBJECTIVE: The aim of the study was to investigate the effect of chemical compounds of Galla chinensis on in vitro the remineralization of enamel surface. METHOD: Bovine enamel blocks with in vitro produced initial lesions were used. The lesions were subjected to a pH-cycling regime for 12 days. Each daily cycle included 4x1 min applications with one of four treatments: Group A: 1000 ppm F aq. (as NaF, positive control); Group B: deionized water (DDW, negative control); Group C: 4000 ppm crude aqueous extract of G. chinensis (GCE); Group D: 4000 ppm gallic acid. The enamel specimens were analysed by scanning electron microscopy (SEM), energy-dispersive X-ray spectrometry (EDS) and X-ray microdiffraction (XRD). RESULTS: The morphology of surface enamel was different in each group. The mineral composition was identified as similar to hydroxylapatite, but the crystallite sizes of enamel in different groups were significantly different. In addition, the Ca/P ratios were significantly lower in GCE group. CONCLUSION: GCE could affect the mineral ions deposit on the surface layer and then modified the remineralization of initial dental caries. The mechanism seems to be different between GCE and gallic acid. Copyright 2010 Elsevier Ltd. All rights reserved.
OBJECTIVE: The aim of the study was to investigate the effect of chemical compounds of Galla chinensis on in vitro the remineralization of enamel surface. METHOD:Bovine enamel blocks with in vitro produced initial lesions were used. The lesions were subjected to a pH-cycling regime for 12 days. Each daily cycle included 4x1 min applications with one of four treatments: Group A: 1000 ppm F aq. (as NaF, positive control); Group B: deionized water (DDW, negative control); Group C: 4000 ppm crude aqueous extract of G. chinensis (GCE); Group D: 4000 ppm gallic acid. The enamel specimens were analysed by scanning electron microscopy (SEM), energy-dispersive X-ray spectrometry (EDS) and X-ray microdiffraction (XRD). RESULTS: The morphology of surface enamel was different in each group. The mineral composition was identified as similar to hydroxylapatite, but the crystallite sizes of enamel in different groups were significantly different. In addition, the Ca/P ratios were significantly lower in GCE group. CONCLUSION: GCE could affect the mineral ions deposit on the surface layer and then modified the remineralization of initial dental caries. The mechanism seems to be different between GCE and gallic acid. Copyright 2010 Elsevier Ltd. All rights reserved.
Authors: Ana Paula Pereira da Silva; Rafael Simões Gonçalves; Ana Flávia Sanches Borges; Ana Karina Bedran-Russo; Mirela Sanae Shinohara Journal: J Appl Oral Sci Date: 2015 May-Jun Impact factor: 2.698