| Literature DB >> 20387637 |
G V Gerashchenko1, V V Gordiyuk, I Ya Skrypkina, S M Kvasha, O O Kolesnik, D D Ugryn, T V Pavlova, E R Zabarovsky, A V Rynditch, V I Kashuba.
Abstract
DNA microarray technology comprising NotI-linking clones was used in a large-scale study of genetic and epigenetic changes in colorectal cancer. Analysis of samples from 24 patients revealed methylation, deletions, and amplifications in 137 of 181 NotI clones. For 27 genes/loci, these changes occurred in more than 30% of the tumor samples, suggesting that these genes are involved in the development of colorectal cancer. An analysis of the methylation status of CpG island of the ITGA9 gene/loci by bisulfite sequencing confirmed the NotI microarray data on the gene/loci methylation in colorectal cancer. Aberrations in 19 genes/loci were unknown previously. Their characterization may help ascertain the mechanisms responsible for colorectal cancer development and identify novel diagnostic and prognostic markers.Entities:
Mesh：
Year: 2009 PMID： 20387637
Source DB: PubMed Journal: Ukr Biokhim Zh (1999)