| Literature DB >> 20387157 |
Abstract
Increasing lines of evidence indicate that small non-coding RNAs including miRNAs, piRNAs, rasiRNAs, 21U endo-siRNAs, and snoRNAs are involved in many critical biological processes. Functional studies of these small RNAs require a simple, sensitive, and reliable method for detecting and quantifying levels of small RNAs. Here, we describe such a method that has been widely used for the validation of cloned small RNAs and also for quantitative analyses of small RNAs in both tissues and cells.Entities:
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Year: 2010 PMID: 20387157 PMCID: PMC3052396 DOI: 10.1007/978-1-60761-657-3_19
Source DB: PubMed Journal: Methods Mol Biol ISSN: 1064-3745