Literature DB >> 20387157

Detection and quantitative analysis of small RNAs by PCR.

Seungil Ro1, Wei Yan.   

Abstract

Increasing lines of evidence indicate that small non-coding RNAs including miRNAs, piRNAs, rasiRNAs, 21U endo-siRNAs, and snoRNAs are involved in many critical biological processes. Functional studies of these small RNAs require a simple, sensitive, and reliable method for detecting and quantifying levels of small RNAs. Here, we describe such a method that has been widely used for the validation of cloned small RNAs and also for quantitative analyses of small RNAs in both tissues and cells.

Entities:  

Mesh:

Substances:

Year:  2010        PMID: 20387157      PMCID: PMC3052396          DOI: 10.1007/978-1-60761-657-3_19

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN: 1064-3745


  40 in total

1.  The small RNA profile during Drosophila melanogaster development.

Authors:  Alexei A Aravin; Mariana Lagos-Quintana; Abdullah Yalcin; Mihaela Zavolan; Debora Marks; Ben Snyder; Terry Gaasterland; Jutta Meyer; Thomas Tuschl
Journal:  Dev Cell       Date:  2003-08       Impact factor: 12.270

Review 2.  MicroRNA biogenesis: coordinated cropping and dicing.

Authors:  V Narry Kim
Journal:  Nat Rev Mol Cell Biol       Date:  2005-05       Impact factor: 94.444

Review 3.  Small RNAs just got bigger: Piwi-interacting RNAs (piRNAs) in mammalian testes.

Authors:  V Narry Kim
Journal:  Genes Dev       Date:  2006-08-01       Impact factor: 11.361

Review 4.  MicroRNA detection by microarray.

Authors:  Wei Li; Kangcheng Ruan
Journal:  Anal Bioanal Chem       Date:  2009-01-09       Impact factor: 4.142

5.  Conservation of the H19 noncoding RNA and H19-IGF2 imprinting mechanism in therians.

Authors:  Guillaume Smits; Andrew J Mungall; Sam Griffiths-Jones; Paul Smith; Delphine Beury; Lucy Matthews; Jane Rogers; Andrew J Pask; Geoff Shaw; John L VandeBerg; John R McCarrey; Marilyn B Renfree; Wolf Reik; Ian Dunham
Journal:  Nat Genet       Date:  2008-06-29       Impact factor: 38.330

6.  An abundant class of tiny RNAs with probable regulatory roles in Caenorhabditis elegans.

Authors:  N C Lau; L P Lim; E G Weinstein; D P Bartel
Journal:  Science       Date:  2001-10-26       Impact factor: 47.728

7.  An extensive class of small RNAs in Caenorhabditis elegans.

Authors:  R C Lee; V Ambros
Journal:  Science       Date:  2001-10-26       Impact factor: 47.728

8.  Sertoli cell Dicer is essential for spermatogenesis in mice.

Authors:  Marilena D Papaioannou; Jean-Luc Pitetti; Seungil Ro; Chanjae Park; Florence Aubry; Olivier Schaad; Charles E Vejnar; Francoise Kühne; Patrick Descombes; Evgeny M Zdobnov; Michael T McManus; Florian Guillou; Brian D Harfe; Wei Yan; Bernard Jégou; Serge Nef
Journal:  Dev Biol       Date:  2008-11-28       Impact factor: 3.582

9.  Many X-linked microRNAs escape meiotic sex chromosome inactivation.

Authors:  Rui Song; Seungil Ro; Jason D Michaels; Chanjae Park; John R McCarrey; Wei Yan
Journal:  Nat Genet       Date:  2009-03-22       Impact factor: 38.330

10.  Non-coding RNA annotation of the genome of Trichoplax adhaerens.

Authors:  Jana Hertel; Danielle de Jong; Manja Marz; Dominic Rose; Hakim Tafer; Andrea Tanzer; Bernd Schierwater; Peter F Stadler
Journal:  Nucleic Acids Res       Date:  2009-01-16       Impact factor: 16.971

View more
  4 in total

1.  Microfluidics-based digital quantitative PCR for single-cell small RNA quantification.

Authors:  Tian Yu; Chong Tang; Ying Zhang; Ruirui Zhang; Wei Yan
Journal:  Biol Reprod       Date:  2017-09-01       Impact factor: 4.285

2.  Small RNAs from the wheat stripe rust fungus (Puccinia striiformis f.sp. tritici).

Authors:  Nicholas A Mueth; Sowmya R Ramachandran; Scot H Hulbert
Journal:  BMC Genomics       Date:  2015-09-21       Impact factor: 3.969

3.  Small RNA repertoires and their intraspecies variation in Aggregatibacter actinomycetemcomitans.

Authors:  Yuichi Oogai; Yasuhiro Gotoh; Yoshitoshi Ogura; Miki Kawada-Matsuo; Tetsuya Hayashi; Hitoshi Komatsuzawa
Journal:  DNA Res       Date:  2018-04-01       Impact factor: 4.458

4.  Resistance to CymMV and ORSV in artificial microRNA transgenic Nicotiana benthamiana plants.

Authors:  Udomporn Petchthai; Celestine Shi Le Yee; Sek-Man Wong
Journal:  Sci Rep       Date:  2018-07-02       Impact factor: 4.379

  4 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.