Literature DB >> 20385179

Accurate and simple sizing of primer extension products using a non-radioactive approach facilitates identification of transcription initiation sites.

Sarah Broadbent1, Marjan van der Woude, Naveed Aziz.   

Abstract

An improved method of non-radioactive identification of transcription start sites is presented in which the use of 7-deaza dGTP in the primer extension reaction allows the product to be directly aligned to cycle sequencing traces on an automated sequencer. This removes the documented need to apply corrections for mobility differences. 2010 Elsevier B.V. All rights reserved.

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Year:  2010        PMID： 20385179     DOI： 10.1016/j.mimet.2010.03.021

Source DB:  PubMed          Journal:  J Microbiol Methods        ISSN： 0167-7012            Impact factor:   2.363


  7 in total

1.  Comprehensive cloning of patient-derived 9022-bp amplicons of hepatitis C virus.

Authors:  Yang Lu; Yanjuan Xu; Adrian M Di Bisceglie; Xiaofeng Fan
Journal:  J Virol Methods       Date:  2013-04-17       Impact factor: 2.014

2.  Transcriptional and epigenetic regulation of KIAA1199 gene expression in human breast cancer.

Authors:  Cem Kuscu; Nikki Evensen; Deborah Kim; You-Jun Hu; Stanley Zucker; Jian Cao
Journal:  PLoS One       Date:  2012-09-06       Impact factor: 3.240

3.  Phase variation controls expression of Salmonella lipopolysaccharide modification genes by a DNA methylation-dependent mechanism.

Authors:  S E Broadbent; M R Davies; M W van der Woude
Journal:  Mol Microbiol       Date:  2010-05-12       Impact factor: 3.501

4.  A BTP1 prophage gene present in invasive non-typhoidal Salmonella determines composition and length of the O-antigen of the lipopolysaccharide.

Authors:  Erica Kintz; Mark R Davies; Disa L Hammarlöf; Rocío Canals; Jay C D Hinton; Marjan W van der Woude
Journal:  Mol Microbiol       Date:  2015-02-11       Impact factor: 3.501

5.  Transcription of Leishmania major U2 small nuclear RNA gene is directed by extragenic sequences located within a tRNA-like and a tRNA-Ala gene.

Authors:  Saúl Rojas-Sánchez; Elisa Figueroa-Angulo; Rodrigo Moreno-Campos; Luis E Florencio-Martínez; Rebeca G Manning-Cela; Santiago Martínez-Calvillo
Journal:  Parasit Vectors       Date:  2016-07-19       Impact factor: 3.876

6.  Mutation of nucleotides around the +1 position of type 3 polymerase III promoters: The effect on transcriptional activity and start site usage.

Authors:  Zongliang Gao; Alex Harwig; Ben Berkhout; Elena Herrera-Carrillo
Journal:  Transcription       Date:  2017-06-09

7.  Horizontally acquired glycosyltransferase operons drive salmonellae lipopolysaccharide diversity.

Authors:  Mark R Davies; Sarah E Broadbent; Simon R Harris; Nicholas R Thomson; Marjan W van der Woude
Journal:  PLoS Genet       Date:  2013-06-20       Impact factor: 5.917
  7 in total

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