Dynamics of augmented soil system containing biphenyl with Dyellaginsengisoli LA-4.

One high efficient biphenyl-degrading strain Dyella ginsengisoli LA-4 was inoculated into biphenyl-contaminated soil for bioaugmentation in this study. The results showed that bioaugmentation could accelerate the startup period of the biphenyl bioremediation process compared with the non-augmented one. PCR-DGGE fingerprints demonstrated that both of the diversity and pattern of microbial community were affected by the addition of strain LA-4 and biphenyl. Biphenyl-utilizing populations gradually increased and become the dominant species. The introduced strain LA-4 could be persistent and co-exist well with the indigenous populations. However, both of the strain LA-4 and indigenous microorganisms in the bioaugmented system would be partially inhibited by Zn(2+) and Ni(2+). This study suggests that it is feasible and potentially useful to remediate biphenyl-contaminated soil using bioaugmentation with D. ginsengisoli LA-4. 2010 Elsevier B.V. All rights reserved.