Literature DB >> 2037226

Heterologous transformation of Cochliobolus lunatus.

M Dermastia1, D Rozman, R Komel.   

Abstract

A DNA mediated transformation system has been developed for the filamentous fungus Cochliobolus lunatus. Transformants were obtained by using plasmid pAN 7-1 carrying the Escherichia coli hygromycin B phosphotransferase gene (hph) fused to an Aspergillus nidulans promoter. The integration of plasmid pAN 7-1 into the fungal genome altered the ability of this microorganism to transform progesterone.

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Year:  1991        PMID: 2037226     DOI: 10.1016/0378-1097(91)90542-i

Source DB:  PubMed          Journal:  FEMS Microbiol Lett        ISSN: 0378-1097            Impact factor:   2.742


  3 in total

1.  Transformation of Cochliobolus lunatus with pUT 720 changes the steroid hydroxylating ability of the fungus.

Authors:  D Rozman; R Komel
Journal:  Curr Genet       Date:  1992-08       Impact factor: 3.886

2.  Altered expression of the steroid bioconverting pathway in pAN 7-1 transformants of Cochliobolus lunatus.

Authors:  D Rozman; R Komel
Journal:  Curr Genet       Date:  1991-11       Impact factor: 3.886

3.  Elimination of by-products in 11 beta-hydroxylation of substance S using Curvularia lunata clones regenerated from NTG-treated protoplasts.

Authors:  D Wilmańska; K Milczarek; A Rumijowska; K Bartnicka; L Sedlaczek
Journal:  Appl Microbiol Biotechnol       Date:  1992-08       Impact factor: 4.813

  3 in total

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