| Literature DB >> 2037063 |
L Wolfram1, T Eitinger, B Friedrich.
Abstract
The high-affinity nickel transporter of Alcaligenes eutrophus H16 is encoded by gene hoxN, which maps within the hydrogenase gene cluster of megaplasmid pHG1. A tripartite gene fusion was constructed, consisting of (i) the Escherichia coli lacZ gene for beta-galactosidase, (ii) a segment encoding an endoproteolytically cleavable peptide, and (iii) the A. eutrophus gene hoxN. An E. coli strain harboring this construct (plasmid pCH307) efficiently produced the corresponding triprotein upon induction. A broad-host-range derivative of pCH307 was shown to complement an A. eutrophus HoxN- mutant.Entities:
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Year: 1991 PMID： 2037063 DOI： 10.1016/0014-5793(91)80565-k
Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124