Ying Wang1, Yu-ting Zhou, Qing Zhao. 1. Department of Biochemistry, Guangzhou Medical College, Guangzhou 510182, China. vio402let@sohu.com
Abstract
AIM: To study the effect of the activation of cannabinoid receptor on the proliferation and apoptosis of hepatoma HepG2 cells. METHODS: HepG2 cells were treated with different doses of the activator of cannabinoid receptor-WIN-55, 212-2(WIN), MTT was used to detect the effects of WIN on HepG2 cells; Flow cytometry (FCM) was used to examine the effects of WIN on the apoptosis of HepG2 cells. Caspase 3 and c-myc proteins were determined by Western blot assay, and caspase 3, 8, 9 activity was measured using a colorimetric method. RESULTS: WIN could inhibit HepG2 cell proliferation and induce cell apoptosis dose-dependently. WIN increased caspase 3 protein levels and caspase 3, 8, 9 activity. CONCLUSION: WIN can induce apoptosis, the effect might be associated with up-regulating expression of caspases protein and down-regulating expression of c-myc protein.
AIM: To study the effect of the activation of cannabinoid receptor on the proliferation and apoptosis of hepatoma HepG2 cells. METHODS: HepG2 cells were treated with different doses of the activator of cannabinoid receptor-WIN-55, 212-2(WIN), MTT was used to detect the effects of WIN on HepG2 cells; Flow cytometry (FCM) was used to examine the effects of WIN on the apoptosis of HepG2 cells. Caspase 3 and c-myc proteins were determined by Western blot assay, and caspase 3, 8, 9 activity was measured using a colorimetric method. RESULTS: WIN could inhibit HepG2 cell proliferation and induce cell apoptosis dose-dependently. WIN increased caspase 3 protein levels and caspase 3, 8, 9 activity. CONCLUSION: WIN can induce apoptosis, the effect might be associated with up-regulating expression of caspases protein and down-regulating expression of c-myc protein.
Authors: Anberitha T Matthews; Jung Hwa Lee; Abdolsamad Borazjani; Lee C Mangum; Xiang Hou; Matthew K Ross Journal: Am J Physiol Cell Physiol Date: 2016-10-26 Impact factor: 4.249