| Literature DB >> 20363540 |
Yong Tang1, Yi-Fan Zhai, Jun-Jian Xiang, Hong Wang, Bin Liu, Chang-Wei Guo.
Abstract
One-step immunochromatographic assay (ICA) has been developed using colloidal gold-labeled monoclonal antibody probe for the rapid detection of lead ions in water samples. The ICA was based on the theory of competitive reactivity, and the results can be easily judged based on the presence or absence of a red colored test line with visual detection. Under optimal conditions, this method shows high detecting sensitivity with a LOD (limit of detection) of 50 ng/ml. Stability test indicates that the immunochromatographic strips are stable for 8 weeks at room temperature. During practical application, nanometer TiO2 is used to enrich the lead ions in water samples. The ICA is successfully applied in the measurement of lead ion concentrations in local water samples, and the results are highly consistent with that of ICP-MS. Detecting lead ions with ICA can be done within 4 min and is very useful for the rapid onsite testing. Crown Copyright 2010. Published by Elsevier Ltd. All rights reserved.Entities:
Mesh：
Substances：
Year: 2010 PMID： 20363540 DOI： 10.1016/j.envpol.2010.03.001
Source DB: PubMed Journal: Environ Pollut ISSN： 0269-7491 Impact factor: 8.071