Literature DB >> 20363268

Metabolomics-driven approach for the improvement of Chinese hamster ovary cell growth: overexpression of malate dehydrogenase II.

William P K Chong1, Satty G Reddy, Faraaz N K Yusufi, Dong-Yup Lee, Niki S C Wong, Chew Kiat Heng, Miranda G S Yap, Ying Swan Ho.   

Abstract

We have established a liquid chromatography-mass spectrometry based metabolomics platform to identify extracellular metabolites in the medium of recombinant Chinese hamster ovary (CHO) fed-batch reactor cultures. Amongst the extracellular metabolites identified, malate accumulation was the most significant. The contributing factors to malate efflux were found to be the supply of aspartate from the medium, and an enzymatic bottleneck at malate dehydrogenase II (MDH II) in the tricarboxylic acid cycle. Subsequent metabolic engineering to overexpress MDH II in CHO resulted in increases in intracellular ATP and NADH, and up to 1.9-fold improvement in integral viable cell number. 2010 Elsevier B.V. All rights reserved.

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Year:  2010        PMID： 20363268     DOI： 10.1016/j.jbiotec.2010.03.018

Source DB:  PubMed          Journal:  J Biotechnol        ISSN： 0168-1656            Impact factor:   3.307


  14 in total

1.  NMR-based metabolomics of mammalian cell and tissue cultures.

Authors:  Nelly Aranibar; Michael Borys; Nancy A Mackin; Van Ly; Nicholas Abu-Absi; Susan Abu-Absi; Matthias Niemitz; Bernhard Schilling; Zheng Jian Li; Barry Brock; Reb J Russell; Adrienne Tymiak; Michael D Reily
Journal:  J Biomol NMR       Date:  2011-03-04       Impact factor: 2.835

Review 2.  Advancement in bioprocess technology: parallels between microbial natural products and cell culture biologics.

Authors:  Arpan A Bandyopadhyay; Anurag Khetan; Li-Hong Malmberg; Weichang Zhou; Wei-Shou Hu
Journal:  J Ind Microbiol Biotechnol       Date:  2017-02-09       Impact factor: 3.346

3.  Validation and identification of reference genes in Chinese hamster ovary cells for Fc-fusion protein production.

Authors:  Xiaonan Ma; Ling Zhang; Luming Zhang; Chenglong Wang; Xiaorui Guo; Yu Yang; Lin Wang; Xiangru Li; Ningning Ma
Journal:  Exp Biol Med (Maywood)       Date:  2020-03-26

4.  Metabolomics profiling of extracellular metabolites in CHO-K1 cells cultured in different types of growth media.

Authors:  Salfarina Ezrina Mohmad-Saberi; Yumi Zuhanis Has-Yun Hashim; Maizirwan Mel; Azura Amid; Raha Ahmad-Raus; Vasila Packeer-Mohamed
Journal:  Cytotechnology       Date:  2012-11-20       Impact factor: 2.058

5.  Improvements in protein production in mammalian cells from targeted metabolic engineering.

Authors:  Anne Richelle; Nathan E Lewis
Journal:  Curr Opin Syst Biol       Date:  2017-06-06

6.  Analysis of microRNA transcription and post-transcriptional processing by Dicer in the context of CHO cell proliferation.

Authors:  Matthias Hackl; Vaibhav Jadhav; Gerald Klanert; Michael Karbiener; Marcel Scheideler; Johannes Grillari; Nicole Borth
Journal:  J Biotechnol       Date:  2014-01-28       Impact factor: 3.307

7.  Designed Amino Acid Feed in Improvement of Production and Quality Targets of a Therapeutic Monoclonal Antibody.

Authors:  Fatemeh Torkashvand; Behrouz Vaziri; Shayan Maleknia; Amir Heydari; Manouchehr Vossoughi; Fatemeh Davami; Fereidoun Mahboudi
Journal:  PLoS One       Date:  2015-10-19       Impact factor: 3.240

8.  Multi-omic profiling -of EPO-producing Chinese hamster ovary cell panel reveals metabolic adaptation to heterologous protein production.

Authors:  Daniel Ley; Ali Kazemi Seresht; Mikael Engmark; Olivera Magdenoska; Kristian Fog Nielsen; Helene Faustrup Kildegaard; Mikael Rørdam Andersen
Journal:  Biotechnol Bioeng       Date:  2015-06-30       Impact factor: 4.530

Review 9.  CHO microRNA engineering is growing up: recent successes and future challenges.

Authors:  Vaibhav Jadhav; Matthias Hackl; Aliaksandr Druz; Smriti Shridhar; Cheng-Yu Chung; Kelley M Heffner; David P Kreil; Mike Betenbaugh; Joseph Shiloach; Niall Barron; Johannes Grillari; Nicole Borth
Journal:  Biotechnol Adv       Date:  2013-08-02       Impact factor: 14.227

10.  Comparative metabolic analysis of CHO cell clones obtained through cell engineering, for IgG productivity, growth and cell longevity.

Authors:  Camila A Wilkens; Ziomara P Gerdtzen
Journal:  PLoS One       Date:  2015-03-13       Impact factor: 3.240
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