Literature DB >> 20361920

High-resolution separation of tubulin monomers on polyacrylamide minigels.

Abhijit Banerjee1, Frank A Bovenzi, Susan L Bane.   

Abstract

High-resolution separation of alpha- and beta-tubulin by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) on minigels can be performed rapidly using simple modifications of the standard Laemmli procedure. Separation of the subunits can be observed even in high-protein loads (up to 40microg of protein). 2010 Elsevier Inc. All rights reserved.

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Year:  2010        PMID： 20361920      PMCID： PMC2885144          DOI： 10.1016/j.ab.2010.03.035

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  16 in total

1.  Preparation and properties of pure tubulin S.

Authors:  L Knipling; J Hwang; J Wolff
Journal:  Cell Motil Cytoskeleton       Date:  1999

2.  High-resolution preparative SDS-polyacrylamide gel electrophoresis: fluorescent visualization and electrophoretic elution-concentration of protein bands.

Authors:  R E Stephens
Journal:  Anal Biochem       Date:  1975-05-12       Impact factor: 3.365

3.  Separation of tubulin isoforms by isoelectric focusing in immobilized pH gradient gels.

Authors:  R C Williams; C Shah; D Sackett
Journal:  Anal Biochem       Date:  1999-11-15       Impact factor: 3.365

4.  Alpha- and beta-tubulin: separation and partial sequence analysis.

Authors:  R F Luduena; D O Woodward
Journal:  Ann N Y Acad Sci       Date:  1975-06-30       Impact factor: 5.691

5.  Interaction of microtubule-associated proteins with microtubules: yeast lysyl- and valyl-tRNA synthetases and tau 218-235 synthetic peptide as model systems.

Authors:  R Melki; P Kerjan; J P Waller; M F Carlier; D Pantaloni
Journal:  Biochemistry       Date:  1991-12-10       Impact factor: 3.162

Review 6.  Multiple forms of tubulin: different gene products and covalent modifications.

Authors:  R F Ludueña
Journal:  Int Rev Cytol       Date:  1998

7.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

8.  Brain and erythrocyte microtubules from chicken contain different beta-tubulin polypeptides.

Authors:  D B Murphy; K T Wallis
Journal:  J Biol Chem       Date:  1983-06-25       Impact factor: 5.157

9.  Electrophoretic resolution of tubulin and tektin subunits by differential interaction with long-chain alkyl sulfates.

Authors:  R E Stephens
Journal:  Anal Biochem       Date:  1998-12-15       Impact factor: 3.365

10.  The two alpha-tubulin isotypes in budding yeast have opposing effects on microtubule dynamics in vitro.

Authors:  Claudia J Bode; Mohan L Gupta; Kathy A Suprenant; Richard H Himes
Journal:  EMBO Rep       Date:  2003-01       Impact factor: 8.807
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  4 in total

1.  The force required to remove tubulin from the microtubule lattice by pulling on its α-tubulin C-terminal tail.

Authors:  Yin-Wei Kuo; Mohammed Mahamdeh; Yazgan Tuna; Jonathon Howard
Journal:  Nat Commun       Date:  2022-06-25       Impact factor: 17.694

2.  Structural basis for polyglutamate chain initiation and elongation by TTLL family enzymes.

Authors:  Kishore K Mahalingan; E Keith Keenan; Madeleine Strickland; Yan Li; Yanjie Liu; Haydn L Ball; Martin E Tanner; Nico Tjandra; Antonina Roll-Mecak
Journal:  Nat Struct Mol Biol       Date:  2020-08-03       Impact factor: 15.369

3.  C-terminal region of MAP7 domain containing protein 3 (MAP7D3) promotes microtubule polymerization by binding at the C-terminal tail of tubulin.

Authors:  Saroj Yadav; Paul J Verma; Dulal Panda
Journal:  PLoS One       Date:  2014-06-13       Impact factor: 3.240

4.  Identification of DmTTLL5 as a Major Tubulin Glutamylase in the Drosophila Nervous System.

Authors:  Isabelle Devambez; Juliette van Dijk; Salim Benlefki; Sophie Layalle; Yves Grau; Krzysztof Rogowski; Marie-Laure Parmentier; Laurent Soustelle
Journal:  Sci Rep       Date:  2017-11-24       Impact factor: 4.379
  4 in total

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