Literature DB >> 2035838

A quantitative microwell assay for chondrocyte cell adhesion.

R R Miller1, C A McDevitt.   

Abstract

An assay for measuring the quantity of live articular chondrocytes attached to a substratum in microwell plates was established by measuring the absorbance of the blue formazan product generated from the dye 3-(4,5-dimethylthiazol-2-yl)-2,5-dypenyl tetrazolium bromide (MTT). Blue formazan production was optimal at an MTT concentration of 1 mg/ml (100 microliters per microwell) and an incubation period of 3 h. The absorbance of the dye was linearly related to the quantity of cells added per microwell. The number of live chondrocytes attached to adhesive proteins can be quantitated using this technique.

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Year:  1991        PMID： 2035838     DOI： 10.1016/0003-2697(91)90552-5

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  4 in total

1.  Herpes simplex virus type 1 latency-associated transcripts suppress viral replication and reduce immediate-early gene mRNA levels in a neuronal cell line.

Authors:  N Mador; D Goldenberg; O Cohen; A Panet; I Steiner
Journal:  J Virol       Date:  1998-06       Impact factor: 5.103

2.  MEF2 negatively regulates learning-induced structural plasticity and memory formation.

Authors:  Christina J Cole; Valentina Mercaldo; Leonardo Restivo; Adelaide P Yiu; Melanie J Sekeres; Jin-Hee Han; Gisella Vetere; Tetyana Pekar; P Joel Ross; Rachael L Neve; Paul W Frankland; Sheena A Josselyn
Journal:  Nat Neurosci       Date:  2012-08-12       Impact factor: 24.884

3.  Assessment of antineoplastic agents by MTT assay: partial underestimation of antiproliferative properties.

Authors:  S B Sobottka; M R Berger
Journal:  Cancer Chemother Pharmacol       Date:  1992       Impact factor: 3.333

4.  Transgenic mouse with the herpes simplex virus type 1 latency-associated gene: expression and function of the transgene.

Authors:  Nurith Mador; Efrat Braun; Hillel Haim; Ilana Ariel; Amos Panet; Israel Steiner
Journal:  J Virol       Date:  2003-12       Impact factor: 5.103
  4 in total

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