BACKGROUND: A water-soluble polysaccharide from the seeds of Plantago asiatica L. (P. asiatica L. polysaccharide, PLP) was extracted with hot water and purified by gel filtration chromatography. The chemical characteristics of PLP were determined by high-performance gel permeation chromatography (HPGPC) and Fourier transform infrared (FTIR) spectroscopy. In addition, the antioxidant activities of PLP in vitro were evaluated using various test systems, including scavenging of 1,1-diphenyl-2-picryl-hydrazyl (DPPH) radicals, scavenging of superoxide radicals generated by 1,2,3-phentriol autoxidation, scavenging of hydroxyl radicals and inhibition of lipid peroxidation. RESULTS: The molecular weight of PLP was determined by HPGPC to be about 1894 kDa. PLP contained 29.2 g kg(-1) protein and 145.8 g kg(-1) uronic acid. The FTIR spectrum of PLP also revealed typical characteristics of a polysaccharide containing protein and uronic acid. Moreover, the results showed that PLP possessed antioxidant activities, but lower than those of ascorbic acid. CONCLUSION: PLP is an acid protein-bound polysaccharide of high molecular weight, but its structure needs further study. The present results suggest that PLP could potentially be used as a natural antioxidant. (c) 2009 Society of Chemical Industry.
BACKGROUND: A water-soluble polysaccharide from the seeds of Plantago asiatica L. (P. asiatica L. polysaccharide, PLP) was extracted with hot water and purified by gel filtration chromatography. The chemical characteristics of PLP were determined by high-performance gel permeation chromatography (HPGPC) and Fourier transform infrared (FTIR) spectroscopy. In addition, the antioxidant activities of PLP in vitro were evaluated using various test systems, including scavenging of 1,1-diphenyl-2-picryl-hydrazyl (DPPH) radicals, scavenging of superoxide radicals generated by 1,2,3-phentriol autoxidation, scavenging of hydroxyl radicals and inhibition of lipid peroxidation. RESULTS: The molecular weight of PLP was determined by HPGPC to be about 1894 kDa. PLP contained 29.2 g kg(-1) protein and 145.8 g kg(-1) uronic acid. The FTIR spectrum of PLP also revealed typical characteristics of a polysaccharide containing protein and uronic acid. Moreover, the results showed that PLP possessed antioxidant activities, but lower than those of ascorbic acid. CONCLUSION: PLP is an acid protein-bound polysaccharide of high molecular weight, but its structure needs further study. The present results suggest that PLP could potentially be used as a natural antioxidant. (c) 2009 Society of Chemical Industry.
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