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Literature DB >> 2034657

Primary structure of a cerulenin-binding beta-ketoacyl-[acyl carrier protein] synthase from barley chloroplasts.

M Siggaard-Andersen¹, S Kauppinen, P von Wettstein-Knowles.

Abstract

The radioactively labeled beta-ketoacyl thioester synthase inhibitor [3H] cerulenin was used to tag three dimeric barely chloroplast proteins (alpha alpha, alpha beta, and beta beta) from the stromal fraction. Oligonucleotides corresponding to amino acid sequences obtained from the purified proteins were used to generate with the polymerase chain reaction a probe for cDNAs encoding the beta subunit. cDNA sequencing revealed an open reading frame for 462 residues comprising the mature protein and a 35-amino acid transit peptide. The deduced amino acid sequence of the mature protein is homologous to the beta-ketoacyl-[acyl carrier protein] (ACP) synthase I [3-oxoacyl-ACP synthase; acyl-ACP:malonyl-ACP C-acyltransferase (decarboxylating), EC 2.3.1.41] of Escherichia coli. Under analogous experimental conditions [3H]cerulenin tagged a single dimeric protein from spinach chloroplasts.

Entities: Chemical Gene Species

Mesh：

Substances：

Year: 1991 PMID： 2034657 PMCID： PMC51608 DOI： 10.1073/pnas.88.10.4114

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

21 in total

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Journal: Anal Biochem Date: 1989-08-15 Impact factor: 3.365

3. Role of Escherichia coli beta-ketoacyl-ACP synthase I in unsaturated fatty acid synthesis.

Authors: M Siggaard-Andersen
Journal: Carlsberg Res Commun Date: 1988

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Journal: Anal Biochem Date: 1988-08-15 Impact factor: 3.365

6. A simple and very efficient method for generating cDNA libraries.

Authors: U Gubler; B J Hoffman
Journal: Gene Date: 1983-11 Impact factor: 3.688

7. Inhibition of fatty acid synthetases by the antibiotic cerulenin.

Authors: D Vance; I Goldberg; O Mitsuhashi; K Bloch
Journal: Biochem Biophys Res Commun Date: 1972-08-07 Impact factor: 3.575

8. Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.

Authors: H Aviv; P Leder
Journal: Proc Natl Acad Sci U S A Date: 1972-06 Impact factor: 11.205

9. Binding site of cerulenin in fatty acid synthetase.

Authors: H Funabashi; A Kawaguchi; H Tomoda; S Omura; S Okuda; S Iwasaki
Journal: J Biochem Date: 1989-05 Impact factor: 3.387

10. The characteristics of some components of the fatty acid synthetase system in the plastids from the mesocarp of avocado (Persea americana) fruit.

Authors: I Caughey; R G Kekwick
Journal: Eur J Biochem Date: 1982-04

18 in total

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Review 2. The biochemistry and molecular biology of plant lipid biosynthesis.

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3. Barley Acyl Carrier Protein II: Nucleotide Sequence of cDNA Clones and Chromosomal Location of the Acl2 Gene.

Authors: L Hansen; S Kauppinen
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4. The barley genes Acl1 and Acl3 encoding acyl carrier proteins I and III are located on different chromosomes.

Authors: L Hansen; P von Wettstein-Knowles
Journal: Mol Gen Genet Date: 1991-10

5. Expression of chalcone synthase, dihydroflavonol reductase, and flavanone-3-hydroxylase in mutants of barley deficient in anthocyanin and proanthocyanidin biosynthesis.

Authors: M Meldgaard
Journal: Theor Appl Genet Date: 1992-04 Impact factor: 5.699

6. Molecular cloning of higher-plant 3-oxoacyl-(acyl carrier protein) reductase. Sequence identities with the nodG-gene product of the nitrogen-fixing soil bacterium Rhizobium meliloti.

Authors: A R Slabas; D Chase; I Nishida; N Murata; C Sidebottom; R Safford; P S Sheldon; R G Kekwick; D G Hardie; R W Mackintosh
Journal: Biochem J Date: 1992-04-15 Impact factor: 3.857

7. A beta-ketoacyl-acyl carrier protein synthase III gene (fabH) is encoded on the chloroplast genome of the red alga Porphyra umbilicalis.

Authors: M Reith
Journal: Plant Mol Biol Date: 1993-01 Impact factor: 4.076