| Literature DB >> 2034226 |
S Taguchi1, K Nishiyama, I Kumagai, H Momose, K Miura.
Abstract
Two sets of the Shine-Dalgarno sequence and the initiation codon (ATG) for translation of a gene encoding the protein SSI (Streptomyces subtilisin inhibitor) were studied in vivo by site-directed mutagenesis. The result shows that each ATG can function as an initiator of translation in either Streptomyces lividans 66 or Escherichia coli. The choice of initiation codon seems dependent on the host strain and is closely related to the processing mechanism of pre-SSI protein. The upstream ATG is presumed to be utilized preferentially giving two cleavage sites in pre-SSI in S. albogriseolus S-3253, the original SSI producer strain.Entities:
Mesh：
Substances：
Year: 1991 PMID： 2034226 DOI： 10.1007/bf00273622
Source DB: PubMed Journal: Mol Gen Genet ISSN： 0026-8925