Literature DB >> 20335419

Evaluation of blood agar microtiter plates for culturing leishmania parasites to titrate parasite burden in spleen and peripheral blood of patients with visceral leishmaniasis.

Radheshyam Maurya1, Sanjana Mehrotra, Vijay Kumar Prajapati, Susanne Nylén, David Sacks, Shyam Sundar.   

Abstract

Serial dilution of blood and spleen biopsy specimens, plated on Novy-MacNeal-Nicolle (NNN) blood agar using microtiter culture plates, is a sensitive and reproducible method for detection and growth of Leishmania parasites. Plates could be easily monitored, and growth could be rapidly detected. Moreover, parasite number may be estimated using this technique.

Entities:  

Mesh：

Substances：

Year:  2010        PMID： 20335419      PMCID： PMC2863905          DOI： 10.1128/JCM.01733-09

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  10 in total

1.  A natural model of Leishmania major infection reveals a prolonged "silent" phase of parasite amplification in the skin before the onset of lesion formation and immunity.

Authors:  Y Belkaid; S Mendez; R Lira; N Kadambi; G Milon; D Sacks
Journal:  J Immunol       Date:  2000-07-15       Impact factor: 5.422

Review 2.  Laboratory diagnosis of visceral leishmaniasis.

Authors:  Shyam Sundar; M Rai
Journal:  Clin Diagn Lab Immunol       Date:  2002-09

3.  Diagnostic lag period in kala-azar: test for early diagnosis needed.

Authors:  S Sundar; K Kumar; V P Singh; T M Mahopatra
Journal:  J Assoc Physicians India       Date:  1991-08

4.  Optimized PCR using patient blood samples for diagnosis and follow-up of visceral Leishmaniasis, with special reference to AIDS patients.

Authors:  L Lachaud; J Dereure; E Chabbert; J Reynes; J M Mauboussin; E Oziol; J P Dedet; P Bastien
Journal:  J Clin Microbiol       Date:  2000-01       Impact factor: 5.948

5.  The potential of latent class analysis in diagnostic test validation for canine Leishmania infantum infection.

Authors:  M Boelaert; K Aoun; J Liinev; E Goetghebeur; P Van der Stuyft
Journal:  Epidemiol Infect       Date:  1999-12       Impact factor: 2.451

6.  Splenic aspiration; experience in Kenya.

Authors:  P A Kager; P H Rees; F M Manguyu; K M Bhatt; S M Bhatt
Journal:  Trop Geogr Med       Date:  1983-06

7.  Quantitation of amastigotes of Leishmania donovani in smears of splenic aspirates from patients with visceral leishmaniasis.

Authors:  J D Chulay; A D Bryceson
Journal:  Am J Trop Med Hyg       Date:  1983-05       Impact factor: 2.345

8.  Kala-azar: a comparative study of parasitological methods and the direct agglutination test in diagnosis.

Authors:  E E Zijlstra; M S Ali; A M el-Hassan; I A el-Toum; M Satti; H W Ghalib; P A Kager
Journal:  Trans R Soc Trop Med Hyg       Date:  1992 Sep-Oct       Impact factor: 2.184

9.  Prevalence of agglutinating anti-Leishmania antibodies in two multi-thousand Bengoli communities.

Authors:  M S Chowdhury; A el Harith; A al Massum; E al Karim; A al Rahman
Journal:  Parasitol Res       Date:  1993       Impact factor: 2.289

10.  A microculture technique for isolating live Leishmania parasites from peripheral blood of visceral leishmaniasis patients.

Authors:  M Hide; R Singh; B Kumar; A L Bañuls; S Sundar
Journal:  Acta Trop       Date:  2007-05-01       Impact factor: 3.112
  10 in total
  22 in total

Review 1.  Diagnosis of visceral leishmaniasis: developments over the last decade.

Authors:  Gurumurthy Srividya; Arpita Kulshrestha; Ruchi Singh; Poonam Salotra
Journal:  Parasitol Res       Date:  2011-11-09       Impact factor: 2.289

Review 2.  Diagnosis of visceral leishmaniasis.

Authors:  Pankaj Srivastava; Anand Dayama; Sanjana Mehrotra; Shyam Sundar
Journal:  Trans R Soc Trop Med Hyg       Date:  2010-11-11       Impact factor: 2.184

3.  A Correlative Study of Splenic Parasite Score and Peripheral Blood Parasite Load Estimation by Quantitative PCR in Visceral Leishmaniasis.

Authors:  Medhavi Sudarshan; Toolika Singh; Jaya Chakravarty; Shyam Sundar
Journal:  J Clin Microbiol       Date:  2015-12       Impact factor: 5.948

4.  In vitro susceptibility of Leishmania donovani to miltefosine in Indian visceral leishmaniasis.

Authors:  Vijay Kumar Prajapati; Smriti Sharma; Madhukar Rai; Bart Ostyn; Poonam Salotra; Manu Vanaerschot; Jean-Claude Dujardin; Shyam Sundar
Journal:  Am J Trop Med Hyg       Date:  2013-08-26       Impact factor: 2.345

Review 5.  Parasitic infections and myositis.

Authors:  Samar N El-Beshbishi; Nairmen N Ahmed; Samar H Mostafa; Goman A El-Ganainy
Journal:  Parasitol Res       Date:  2011-09-01       Impact factor: 2.289

6.  Study of parasite kinetics with antileishmanial drugs using real-time quantitative PCR in Indian visceral leishmaniasis.

Authors:  Medhavi Sudarshan; Jason L Weirather; Mary E Wilson; Shyam Sundar
Journal:  J Antimicrob Chemother       Date:  2011-05-24       Impact factor: 5.790

Review 7.  Molecular Diagnosis of Visceral Leishmaniasis.

Authors:  Shyam Sundar; Om Prakash Singh
Journal:  Mol Diagn Ther       Date:  2018-08       Impact factor: 4.074

8.  Antileishmanial activity of cryptolepine analogues and apoptotic effects of 2,7-dibromocryptolepine against Leishmania donovani promastigotes.

Authors:  Sudipta Hazra; Subhalakshmi Ghosh; Sukalyani Debnath; Scott Seville; Vijay Kumar Prajapati; Colin W Wright; Shyam Sundar; Banasri Hazra
Journal:  Parasitol Res       Date:  2012-07       Impact factor: 2.289

9.  Serial quantitative PCR assay for detection, species discrimination, and quantification of Leishmania spp. in human samples.

Authors:  Jason L Weirather; Selma M B Jeronimo; Shalini Gautam; Shyam Sundar; Mitchell Kang; Melissa A Kurtz; Rashidul Haque; Albert Schriefer; Sinésio Talhari; Edgar M Carvalho; John E Donelson; Mary E Wilson
Journal:  J Clin Microbiol       Date:  2011-11       Impact factor: 5.948

10.  An oral formulation of amphotericin B attached to functionalized carbon nanotubes is an effective treatment for experimental visceral leishmaniasis.

Authors:  Vijay Kumar Prajapati; Kalpna Awasthi; Thakur Prasad Yadav; Madhukar Rai; Onkar Nath Srivastava; Shyam Sundar
Journal:  J Infect Dis       Date:  2011-12-12       Impact factor: 5.226
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.