Literature DB >> 20333479

Derivation and characterisation of the human embryonic stem cell line, OxF1.

Frances Anne Brook1, Sally A Cowley, Edward P Evans, Karen Turner, William S James, Helen J Mardon.   

Abstract

OxF1 is a human embryonic stem cell line derived from a surplus embryo donated through the Oxford IVF clinic. The cells have a stable 46 XX karyotype and show expression of Oct 4, Nanog and TRA-1-60. Embryoid bodies differentiate into cells that represent all three germ layers as demonstrated by immunohistochemical localisation of beta III tubulin, nestin, desmin, smooth muscle actin, Gata 6 and cytokeratin 18. Directed differentiation through haematopoiesis has been demonstrated.

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Year:  2010        PMID： 20333479     DOI： 10.1007/s11626-010-9283-5

Source DB:  PubMed          Journal:  In Vitro Cell Dev Biol Anim        ISSN： 1071-2690            Impact factor:   2.416


  2 in total

1.  Mechanical isolation of the inner cell mass is effective in derivation of new human embryonic stem cell lines.

Authors:  Susanne Ström; José Inzunza; Karl-Henrik Grinnemo; Kerstin Holmberg; Eija Matilainen; Anne-Marie Strömberg; Elisabeth Blennow; Outi Hovatta
Journal:  Hum Reprod       Date:  2007-10-24       Impact factor: 6.918

2.  Homogeneous monocytes and macrophages from human embryonic stem cells following coculture-free differentiation in M-CSF and IL-3.

Authors:  Karl R Karlsson; Sally Cowley; Fernando O Martinez; Michael Shaw; Stephen L Minger; William James
Journal:  Exp Hematol       Date:  2008-06-11       Impact factor: 3.084
  2 in total
  1 in total

Review 1.  Substrates and supplements for hESCs: a critical review.

Authors:  Melisa Candela Crocco; Nilo Fratnz; Adriana Bos-Mikich
Journal:  J Assist Reprod Genet       Date:  2013-01-04       Impact factor: 3.412
  1 in total

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