Literature DB >> 2032722

PCR primers for human chromosomes: reagents for the rapid analysis of somatic cell hybrids.

S Theune1, J Fung, S Todd, A Y Sakaguchi, S L Naylor.   

Abstract

Rapid analysis of somatic cell hybrids can be facilitated by using the polymerase chain reaction (PCR) to assay for genes assigned to specific human chromosomes. We describe PCR primer pairs for genes on the short and long arms of the 22 autosomes and the X chromosome. Some of the primers were designed from the 3' untranslated region of cDNA sequences, whereas others were derived from genomic sequence. Each primer set was tested for its specificity and mapped to a chromosome by screening a somatic cell hybrid panel. Two of the primer pairs (APOC2 and G6PD) detect CA dinucleotide repeat polymorphisms.

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Year:  1991        PMID： 2032722     DOI： 10.1016/0888-7543(91)90418-e

Source DB:  PubMed          Journal:  Genomics        ISSN： 0888-7543            Impact factor:   5.736


  16 in total

1.  Localization of a DNA repair gene (XRCC5) involved in double-strand-break rejoining to human chromosome 2.

Authors:  P A Jeggo; M Hafezparast; A F Thompson; B C Broughton; G P Kaur; M Z Zdzienicka; R S Athwal
Journal:  Proc Natl Acad Sci U S A       Date:  1992-07-15       Impact factor: 11.205

2.  Dinucleotide repeat polymorphism in the human tubulin alpha 1 (testis specific) gene (TUBA1).

Authors:  S Todd; S L Naylor
Journal:  Nucleic Acids Res       Date:  1991-07-11       Impact factor: 16.971

3.  Dinucleotide repeat polymorphism in the human surfactant-associated protein 3 gene (SFTP3).

Authors:  S Todd; S L Naylor
Journal:  Nucleic Acids Res       Date:  1991-07-11       Impact factor: 16.971

4.  Neural-specific expression, genomic structure, and chromosomal localization of the gene encoding the zinc-finger transcription factor NGFI-C.

Authors:  S D Crosby; R A Veile; H Donis-Keller; J M Baraban; R V Bhat; K S Simburger; J Milbrandt
Journal:  Proc Natl Acad Sci U S A       Date:  1992-05-15       Impact factor: 11.205

5.  Mapping of loci and translocation breakpoints in Xq13: isolation of a conserved locus that maps close to CCG1 in human and mouse.

Authors:  V Reed; S H Laval; V McCabe; H F Willard; Y Boyd
Journal:  Mamm Genome       Date:  1994-04       Impact factor: 2.957

6.  Deleted chromosome 20 from a patient with Alagille syndrome isolated in a cell hybrid through leucine transport selection: study of three candidate genes.

Authors:  J F Deleuze; S Dhorne; J Hazan; E Borghi; N Raynaud; N Pollet; M Meunier-Rotival; J Deschatrette; D Alagille; M Hadchouel
Journal:  Mamm Genome       Date:  1994-11       Impact factor: 2.957

7.  A tumor suppressor locus within 3p14-p12 mediates rapid cell death of renal cell carcinoma in vivo.

Authors:  Y Sanchez; A el-Naggar; S Pathak; A M Killary
Journal:  Proc Natl Acad Sci U S A       Date:  1994-04-12       Impact factor: 11.205

8.  Mouse UDP-GlcNAc: dolichyl-phosphate N-acetylglucosaminephosphotransferase. Molecular cloning of the cDNA, generation of anti-peptide antibodies and chromosomal localization.

Authors:  B Rajput; J Ma; N Muniappa; L Schantz; S L Naylor; P A Lalley; I K Vijay
Journal:  Biochem J       Date:  1992-08-01       Impact factor: 3.857

9.  Human adhalin is alternatively spliced and the gene is located on chromosome 17q21.

Authors:  E M McNally; M Yoshida; Y Mizuno; E Ozawa; L M Kunkel
Journal:  Proc Natl Acad Sci U S A       Date:  1994-10-11       Impact factor: 11.205

10.  A radiation hybrid map of the distal short arm of human chromosome 11, containing the Beckwith-Wiedemann and associated embryonal tumor disease loci.

Authors:  C W Richard; M Boehnke; D J Berg; J H Lichy; T C Meeker; E Hauser; R M Myers; D R Cox
Journal:  Am J Hum Genet       Date:  1993-05       Impact factor: 11.025
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