Literature DB >> 2032315

The biotin-(strept)avidin system: principles and applications in biotechnology.

E P Diamandis1, T K Christopoulos.   

Abstract

The biotin-(strept)avidin system has been used for many years in a variety of different applications. Here we present a general overview of the system, describe its components and advantages, and show how the system is used in various applications, with emphasis on immunological and nucleic acid hybridization assays. This system is now considered a versatile independent technology with broad applications in many branches of biotechnology. Clearly, its use will continue to grow in the years to come.

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Year:  1991        PMID: 2032315

Source DB:  PubMed          Journal:  Clin Chem        ISSN: 0009-9147            Impact factor:   8.327


  97 in total

1.  Streptavidin aptamers: affinity tags for the study of RNAs and ribonucleoproteins.

Authors:  C Srisawat; D R Engelke
Journal:  RNA       Date:  2001-04       Impact factor: 4.942

2.  T7 RNA polymerase as a self-replicating label for antigen quantification.

Authors:  Bakhos A Tannous; Eleftheria Laios; Theodore K Christopoulos
Journal:  Nucleic Acids Res       Date:  2002-12-15       Impact factor: 16.971

3.  mRNAs biotinylated within the 5' cap and protected against decapping: new tools to capture RNA-protein complexes.

Authors:  Sylwia Bednarek; Vanesa Madan; Pawel J Sikorski; Ralf Bartenschlager; Joanna Kowalska; Jacek Jemielity
Journal:  Philos Trans R Soc Lond B Biol Sci       Date:  2018-11-05       Impact factor: 6.237

4.  Genome-wide Mapping of Drug-DNA Interactions in Cells with COSMIC (Crosslinking of Small Molecules to Isolate Chromatin).

Authors:  Graham S Erwin; Matthew P Grieshop; Devesh Bhimsaria; Asuka Eguchi; José A Rodríguez-Martínez; Aseem Z Ansari
Journal:  J Vis Exp       Date:  2016-01-20       Impact factor: 1.355

5.  Metabolic biotinylation of recombinant antibody by biotin ligase retained in the endoplasmic reticulum.

Authors:  Bhaswati Barat; Anna M Wu
Journal:  Biomol Eng       Date:  2007-02-15

6.  Cellular uptake of fluorescent labelled biotin-streptavidin microspheres.

Authors:  M Bradley; L Alexander; R M Sanchez-Martin
Journal:  J Fluoresc       Date:  2008-03-11       Impact factor: 2.217

7.  Identification of Group B Streptococcus Capsule Type by Use of a Dual Phenotypic/Genotypic Assay.

Authors:  Areej Alhhazmi; Armaan Pandey; Gregory J Tyrrell
Journal:  J Clin Microbiol       Date:  2017-06-14       Impact factor: 5.948

8.  Biotinylated single-chain variable fragment-based enzyme-linked immunosorbent assay for glycocholic acid.

Authors:  Xiping Cui; Natalia Vasylieva; Ding Shen; Bogdan Barnych; Jun Yang; Qiyi He; Zhengyun Jiang; Suqing Zhao; Bruce D Hammock
Journal:  Analyst       Date:  2018-04-30       Impact factor: 4.616

9.  Protein-protein interaction detection in vitro and in cells by proximity biotinylation.

Authors:  Marta Fernández-Suárez; T Scott Chen; Alice Y Ting
Journal:  J Am Chem Soc       Date:  2008-06-27       Impact factor: 15.419

10.  Recombinant streptavidin fusion proteins as signal reporters in rapid test of human hepatitis C virus infection.

Authors:  Shengliang Zhou; Shinian Cao; Guoliang Ma; Tielin Ding; Jingjing Mu; Weilu Han; Dongxu Sun; Caifa Chen
Journal:  J Clin Lab Anal       Date:  2018-10-23       Impact factor: 2.352

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