INTRODUCTION: Lung transplantation has become the mainstay therapy for patients with end-stage lung disease refractory to medical management. However, the number of patients listed for lung transplantation largely exceeds available donors. The study of lung preservation requires accurate, cost-effective small animal models. We have described a model of ex vivo rat lung perfusion using a commercially available system. METHODS: Male Wistar rats weighing 250 g-300 g were anesthetized with intraperitoneal sodium thiopental (50 mg/kg body weight). The surgical technique included heart-lung block extraction, assembly, and preparation for perfusion and data collection. We used an IL-2 Isolated Perfused Rat or Guinea Pig Lung System (Harvard Apparatus, Holliston, Mass, United States; Hugo Sachs Elektronik, Alemanha). RESULTS: Preliminary results included hemodynamic and pulmonary mechanics data gathered in the experiments. CONCLUSION: The isolated rat lung perfusion system is a reliable method to assess lung preservation. Copyright (c) 2010 Elsevier Inc. All rights reserved.
INTRODUCTION: Lung transplantation has become the mainstay therapy for patients with end-stage lung disease refractory to medical management. However, the number of patients listed for lung transplantation largely exceeds available donors. The study of lung preservation requires accurate, cost-effective small animal models. We have described a model of ex vivo rat lung perfusion using a commercially available system. METHODS: Male Wistar rats weighing 250 g-300 g were anesthetized with intraperitoneal sodium thiopental (50 mg/kg body weight). The surgical technique included heart-lung block extraction, assembly, and preparation for perfusion and data collection. We used an IL-2 Isolated Perfused Rat or Guinea Pig Lung System (Harvard Apparatus, Holliston, Mass, United States; Hugo Sachs Elektronik, Alemanha). RESULTS: Preliminary results included hemodynamic and pulmonary mechanics data gathered in the experiments. CONCLUSION: The isolated rat lung perfusion system is a reliable method to assess lung preservation. Copyright (c) 2010 Elsevier Inc. All rights reserved.
Authors: Kevin Nelson; Christopher Bobba; Emre Eren; Tyler Spata; Malak Tadres; Don Hayes; Sylvester M Black; Samir Ghadiali; Bryan A Whitson Journal: J Vis Exp Date: 2015-02-25 Impact factor: 1.355
Authors: Judith E van Zanden; Henri G D Leuvenink; Erik A M Verschuuren; Michiel E Erasmus; Maximilia C Hottenrott Journal: PLoS One Date: 2021-12-02 Impact factor: 3.240