| Literature DB >> 20304080 |
D H Guo1, D B Sun, R Wu, H M Yang, J S Zheng, C L Fan, B Sun, J F Wang.
Abstract
A serodiagnostic ELISA (rL-ELISA) using recombinant truncated leukotoxin protein PL2 (aa 311-644) of Fusobacterium necrophorum as antigen was developed for detection of antibodies against F. necrophorum from cattle footrot. In rL-ELISA, the recombinant diagnostic antigen showed no cross-reaction with antisera against bovine foot and mouth disease virus, bovine rhinotracheitis virus, bovine viral diarrhea virus, bovine rotavirus type A, bovine Escherichia coli, and bovine Salmonella. The rL-ELISA could confirm the existence of antibodies against F. necrophorum at day 7 after infection. Detection of the field samples indicated relative sensitivity of rL-ELISA to nL-ELISA using the purified native leukotoxin A as antigen was 96.43%, and relative specificity of rL-ELISA to nL-ELISA was 94.26%. These data demonstrated the rL-ELISA would have a potential use for early diagnosis of cattle footrot caused by F. necrophorum. Copyright (c) 2010 Elsevier Ltd. All rights reserved.Entities:
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Year: 2010 PMID： 20304080 DOI： 10.1016/j.anaerobe.2010.03.007
Source DB: PubMed Journal: Anaerobe ISSN： 1075-9964 Impact factor: 3.331