| Literature DB >> 20304011 |
Florian Krammer1, Theresa Schinko, Paul Messner, Dieter Palmberger, Boris Ferko, Reingard Grabherr.
Abstract
Various virus-like particles (VLPs) have been shown to induce cytotoxic T-cell immune response as well as B-cell immune response. This makes VLPs promising candidates for antigen-carrier platforms for various epitopes. Influenza A VLPs were produced displaying a 20 amino acid sequence from Mycobacterium tuberculosis early secretory antigenic target 6 protein (ESAT-6). As this sequence is known to comprise a potent T-cell epitope it was chosen as a model for a foreign epitope to be presented on an influenza VLP scaffold. The ESAT-6 epitope was engineered into the antigenic region B of the influenza hemagglutinin (HA) from strain A/New Caledonia/20/99. VLPs were expressed in insect cells and subjected to immunization studies in mice. High serum antibody titers detected against recombinant ESAT-6 demonstrated the feasibility of influenza A VLPs serving as an efficient platform for epitope presentation. Copyright 2010 Elsevier B.V. All rights reserved.Entities:
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Year: 2010 PMID： 20304011 PMCID： PMC4388402 DOI： 10.1016/j.jviromet.2010.03.003
Source DB: PubMed Journal: J Virol Methods ISSN： 0166-0934 Impact factor: 2.014