BACKGROUND: We aimed to evaluate the total antioxidant capacity (TAC) of saliva in healthy Helicobacter pylori-positive and negative saliva individuals. MATERIALS AND METHODS: A total of 102 human saliva samples were checked for the presence of H. pylori DNA (ureA and cagA gene fragments). TAC of saliva was estimated by ABTS radical cation (ABTS( +)) decolorization assay. RESULTS: PCR analysis revealed that 36 subjects were ureA-/cagA-, 24 were ureA+/cagA- and 42 were ureA+/cagA+. Smoking habits had no evident effect on H. pylori infection. We found that TAC of the ureA-/cagA- material, after 10 seconds reaction reflecting fast-reacting antioxidants, was significantly higher than of ureA+/cagA- and ureA+/cagA+ samples (p < .01 and p < .001, respectively). Similar results were obtained for reaction time of 3 minutes measuring slow-reacting antioxidants (p < .001). We also estimated ureA+/cagA- and ureA+/cagA+ samples alone and reported a statistically significant decrease in the TAC(3 min) value of ureA+/cagA+ compared with ureA+/cagA- samples (p < .05). CONCLUSIONS: Our data demonstrated that altered redox equilibrium may be associated with more frequent occurrence of H. pylori in the saliva samples.
BACKGROUND: We aimed to evaluate the total antioxidant capacity (TAC) of saliva in healthy Helicobacter pylori-positive and negative saliva individuals. MATERIALS AND METHODS: A total of 102 human saliva samples were checked for the presence of H. pylori DNA (ureA and cagA gene fragments). TAC of saliva was estimated by ABTS radical cation (ABTS( +)) decolorization assay. RESULTS: PCR analysis revealed that 36 subjects were ureA-/cagA-, 24 were ureA+/cagA- and 42 were ureA+/cagA+. Smoking habits had no evident effect on H. pyloriinfection. We found that TAC of the ureA-/cagA- material, after 10 seconds reaction reflecting fast-reacting antioxidants, was significantly higher than of ureA+/cagA- and ureA+/cagA+ samples (p < .01 and p < .001, respectively). Similar results were obtained for reaction time of 3 minutes measuring slow-reacting antioxidants (p < .001). We also estimated ureA+/cagA- and ureA+/cagA+ samples alone and reported a statistically significant decrease in the TAC(3 min) value of ureA+/cagA+ compared with ureA+/cagA- samples (p < .05). CONCLUSIONS: Our data demonstrated that altered redox equilibrium may be associated with more frequent occurrence of H. pylori in the saliva samples.