BACKGROUND: Biochemical diagnostics of ethanol intake would improve alcohol abuse treatment and have applications in clinical trial and public safety settings. Self-reporting of alcohol use has clinical utility but lacks the desired reliability. Previously, proposed single-analyte biochemical tests of alcohol intake suffer from low sensitivity and specificity or examine only acute drinking and have therefore seen limited clinical use. METHODS: To address this unmet need, plasma protein biomarker discovery and validation were performed with an alcohol self-administering nonhuman primate model system to develop a diagnostic that accurately classifies subjects into nondrinking, nonabusive drinking, and abusive drinking categories. RESULTS: A 17-plasma protein panel was determined that correctly classifies abusive drinking with 100% sensitivity and also differentiates any level of drinking from alcohol abstinence with 88% accuracy. CONCLUSIONS: The biomarker panel reflects changes in multiple organ systems and suggests robust changes in the plasma proteome with drinking that might serve as a sensitive and specific diagnostic test. The specific plasma proteins altered with alcohol self-administration might represent indicators of alcohol-induced stress on a variety of organ systems. Copyright 2010 Society of Biological Psychiatry. Published by Elsevier Inc. All rights reserved.
BACKGROUND: Biochemical diagnostics of ethanol intake would improve alcohol abuse treatment and have applications in clinical trial and public safety settings. Self-reporting of alcohol use has clinical utility but lacks the desired reliability. Previously, proposed single-analyte biochemical tests of alcohol intake suffer from low sensitivity and specificity or examine only acute drinking and have therefore seen limited clinical use. METHODS: To address this unmet need, plasma protein biomarker discovery and validation were performed with an alcohol self-administering nonhuman primate model system to develop a diagnostic that accurately classifies subjects into nondrinking, nonabusive drinking, and abusive drinking categories. RESULTS: A 17-plasma protein panel was determined that correctly classifies abusive drinking with 100% sensitivity and also differentiates any level of drinking from alcohol abstinence with 88% accuracy. CONCLUSIONS: The biomarker panel reflects changes in multiple organ systems and suggests robust changes in the plasma proteome with drinking that might serve as a sensitive and specific diagnostic test. The specific plasma proteins altered with alcohol self-administration might represent indicators of alcohol-induced stress on a variety of organ systems. Copyright 2010 Society of Biological Psychiatry. Published by Elsevier Inc. All rights reserved.
Authors: Priscilla Ivester; L Jackson Roberts; Tracey Young; Diana Stafforini; Jeffrey Vivian; Cynthia Lees; Jennifer Young; James Daunais; David Friedman; Richard A Rippe; Christopher J Parsons; Kathleen A Grant; Carol Cunningham Journal: Alcohol Clin Exp Res Date: 2007-01 Impact factor: 3.455
Authors: Kathleen A Grant; Xiaoyan Leng; Heather L Green; Kendall T Szeliga; Laura S M Rogers; Steven W Gonzales Journal: Alcohol Clin Exp Res Date: 2008-08-12 Impact factor: 3.455
Authors: Donald W Floyd; David P Friedman; James B Daunais; Peter J Pierre; Kathleen A Grant; Brian A McCool Journal: J Pharmacol Exp Ther Date: 2004-07-27 Impact factor: 4.030
Authors: Christa M Helms; Ilhem Messaoudi; Sophia Jeng; Willard M Freeman; Kent E Vrana; Kathleen A Grant Journal: Alcohol Clin Exp Res Date: 2011-12-05 Impact factor: 3.455
Authors: Katie M Lebold; Kathleen A Grant; Willard M Freeman; Kristine M Wiren; Galen W Miller; Caitlin Kiley; Scott W Leonard; Maret G Traber Journal: Alcohol Clin Exp Res Date: 2010-11-30 Impact factor: 3.455
Authors: Willard M Freeman; Heather D Vanguilder; Elizabeth Guidone; John H Krystal; Kathleen A Grant; Kent E Vrana Journal: Int J Neuropsychopharmacol Date: 2011-02-08 Impact factor: 5.176
Authors: Harshica Fernando; John E Wiktorowicz; Kizhake V Soman; Bhupendra S Kaphalia; M Firoze Khan; G A Shakeel Ansari Journal: Toxicol Appl Pharmacol Date: 2012-11-29 Impact factor: 4.219
Authors: James B Daunais; April T Davenport; Christa M Helms; Steven W Gonzales; Scott E Hemby; David P Friedman; Jonathan P Farro; Erich J Baker; Kathleen A Grant Journal: Alcohol Clin Exp Res Date: 2014-06-18 Impact factor: 3.455