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Literature DB >> 202957

Characterization of early simian virus 40 transcriptional complexes: late transcription in the absence of detectable DNA replication.

Abstract

Isolation of early viral transcriptional complexes and incorporation in vitro of radiolabeled precursors into nascent RNA has permitted an analysis of early simian virus 40 (SV40) transcription. Under conditions such that viral DNA replication was undetectable, both early and late SV40 RNA were synthesized. This finding provides evidence that viral DNA replication is not an absolute requirement for late transcription and supports earlier observations that late viral RNA is synthesized in SV40-infected nonpermissive mouse cells. The majority of the early viral transcriptional activity can be solubilized, indicating that a substantial portion of this RNA is transcribed from free rather than integrated templates. Sedimentation analysis of the transcriptional complexes resulted in the detection of two separate peaks of activity, suggesting the possibility of two distinct types of early SV40 templates.

Entities: Disease Species

Mesh：

Substances：
DNA, Viral
RNA, Viral

Year: 1977 PMID： 202957 PMCID： PMC431756 DOI： 10.1073/pnas.74.12.5443

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

28 in total

1. Synthesis and characterization of late lytic simian virus 40 RNA from transcriptional complexes.

Authors: F J Ferdinand; M Brown; G Khoury
Journal: Virology Date: 1977-05-01 Impact factor: 3.616

2. The control of SV40 transcription during a lytic infection: late RNA synthesis in the presence of inhibitors of DNA replication.

Authors: L J Rosenthal; M Brown
Journal: Nucleic Acids Res Date: 1977-03 Impact factor: 16.971

3. Transcription of simian virus 40. VI. SV 40 DNA-RNA polymerase complex isolated from productively infected cells transcribed in vitro.

Authors: O Laub; Y Aloni
Journal: Virology Date: 1976-12 Impact factor: 3.616

4. Autoregulation of simian virus 40 gene A by T antigen.

Authors: S I Reed; G R Stark; J C Alwine
Journal: Proc Natl Acad Sci U S A Date: 1976-09 Impact factor: 11.205

5. Isolation and characterization of simian virus 40 ribonucleic acid.

Authors: R A Weinberg; S O Warnaar; E Winocour
Journal: J Virol Date: 1972-08 Impact factor: 5.103

6. Transcription of Simian virus 40. I. Separation of the strands of SV40 DNA and hybridization of the separated strands to RNA extracted from lytically infected and transformed cells.

Authors: J Sambrook; P A Sharp; W Keller
Journal: J Mol Biol Date: 1972-09-14 Impact factor: 5.469

7. Extensive symmetrical transcription of Simian Virus 40 DNA in virus-yielding cells.

Authors: Y Aloni
Journal: Proc Natl Acad Sci U S A Date: 1972-09 Impact factor: 11.205

8. Regulation of early and late simian virus 40 transcription: overproduction of early viral RNA in the absence of a functional T-antigen.

Authors: G Khoury; E May
Journal: J Virol Date: 1977-07 Impact factor: 5.103

3. Control elements situated downstream of the major transcriptional start site are sufficient for highly efficient polyomavirus late transcription.

Authors: B Bourachot; M Yaniv; P Herbomel
Journal: J Virol Date: 1989-06 Impact factor: 5.103

4. Activity of simian virus 40 late promoter elements in the absence of large T antigen: evidence for repression of late gene expression.

Authors: J C Alwine; J Picardi
Journal: J Virol Date: 1986-11 Impact factor: 5.103

5. Evidence of transcription from the late region of the integrated simian virus 40 genome in transformed cells: location of the 5' ends of late transcripts in cells abortively infected and in cells transformed by simian virus 40.

Authors: M Ernoult-Lange; E May
Journal: J Virol Date: 1983-06 Impact factor: 5.103

9. Expression and stabilization of microinjected plasmids containing the herpes simplex virus thymidine kinase gene and polyoma virus DNA in mouse cells.

Authors: M Yamaizumi; A L Horwich; F H Ruddle
Journal: Mol Cell Biol Date: 1983-04 Impact factor: 4.272

10. Initiation of transcription by RNA polymerase II in permeable, SV40-infected or noninfected, CVI cells; evidence for multiple promoters of SV40 late transcription.

Authors: R Contreras; W Fiers
Journal: Nucleic Acids Res Date: 1981-01-24 Impact factor: 16.971

Characterization of early simian virus 40 transcriptional complexes: late transcription in the absence of detectable DNA replication.

1. Synthesis and characterization of late lytic simian virus 40 RNA from transcriptional complexes.

2. The control of SV40 transcription during a lytic infection: late RNA synthesis in the presence of inhibitors of DNA replication.

3. Transcription of simian virus 40. VI. SV 40 DNA-RNA polymerase complex isolated from productively infected cells transcribed in vitro.

4. Autoregulation of simian virus 40 gene A by T antigen.

5. Isolation and characterization of simian virus 40 ribonucleic acid.

6. Transcription of Simian virus 40. I. Separation of the strands of SV40 DNA and hybridization of the separated strands to RNA extracted from lytically infected and transformed cells.

7. Extensive symmetrical transcription of Simian Virus 40 DNA in virus-yielding cells.

8. Regulation of early and late simian virus 40 transcription: overproduction of early viral RNA in the absence of a functional T-antigen.

9. Transcription of simian virus 40. 3. Mapping of "early" and "late" species of RNA.

10. Strand orientation of simian virus 40 transcription in productively infected cells.

1. Transformation of human embryonic kidney cells by human papovarirus BK.

2. Extent of transcription of the E strand of polyoma virus DNA during the early phase of productive infection.

3. Control elements situated downstream of the major transcriptional start site are sufficient for highly efficient polyomavirus late transcription.

4. Activity of simian virus 40 late promoter elements in the absence of large T antigen: evidence for repression of late gene expression.

5. Evidence of transcription from the late region of the integrated simian virus 40 genome in transformed cells: location of the 5' ends of late transcripts in cells abortively infected and in cells transformed by simian virus 40.

6. Effect of a tsA mutation of simian virus 40 late gene expression: variations between host cell lines.

7. Association of simian virus 40 T antigen with replicating nucleoprotein complexes of simian virus 40.

8. Extraction of transcribing SV40 chromosomes in very low salt.

9. Expression and stabilization of microinjected plasmids containing the herpes simplex virus thymidine kinase gene and polyoma virus DNA in mouse cells.

10. Initiation of transcription by RNA polymerase II in permeable, SV40-infected or noninfected, CVI cells; evidence for multiple promoters of SV40 late transcription.