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Literature DB >> 2027783

Efficient double stranded sequencing of cDNA clones containing long poly(A) tails using anchored poly(dT) primers.

A S Khan¹, A S Wilcox, J A Hopkins, J M Sikela.

Abstract

Entities: Chemical

Mesh：

Substances：
Poly A
Poly T
DNA

Year: 1991 PMID： 2027783 PMCID： PMC333944 DOI： 10.1093/nar/19.7.1715

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

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2. Oligo(dT) primer generates a high frequency of truncated cDNAs through internal poly(A) priming during reverse transcription.

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Journal: Proc Natl Acad Sci U S A Date: 2002-04-23 Impact factor: 11.205

3. Use of 3' untranslated sequences of human cDNAs for rapid chromosome assignment and conversion to STSs: implications for an expression map of the genome.

Authors: A S Wilcox; A S Khan; J A Hopkins; J M Sikela
Journal: Nucleic Acids Res Date: 1991-04-25 Impact factor: 16.971

Efficient double stranded sequencing of cDNA clones containing long poly(A) tails using anchored poly(dT) primers.

1. A common language for physical mapping of the human genome.

2. Supercoil sequencing: a fast and simple method for sequencing plasmid DNA.

1. Generation of longer cDNA fragments from serial analysis of gene expression tags for gene identification.

2. Oligo(dT) primer generates a high frequency of truncated cDNAs through internal poly(A) priming during reverse transcription.

3. Use of 3' untranslated sequences of human cDNAs for rapid chromosome assignment and conversion to STSs: implications for an expression map of the genome.

4. Restriction landmark cDNA scanning (RLCS): a novel cDNA display system using two-dimensional gel electrophoresis.

5. Screening poly(dA/dT)- cDNAs for gene identification.

Review 6. cDNA phage display for the discovery of theranostic autoantibodies in rheumatoid arthritis.

7. Finding and mapping new genes faster than ever: revisited.