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Literature DB >> 2027748

Hypersensitive site 4 of the human beta globin locus control region.

S Pruzina¹, O Hanscombe, D Whyatt, F Grosveld, S Philipsen.

Abstract

The Locus Control Region (LCR) of the human beta globin gene domain is defined by four erythroid-specific DNasel hypersensitive sites (HSS) located upstream of this multigene cluster. The LCR confers copy number dependent high levels of erythroid specific expression to a linked transgene, independent of the site of integration. To assess the role of the individual hypersensitive sites of the LCR, we have localized HSS4 to a 280bp fragment that is functional both in murine erythroleukaemia (MEL) cells and in transgenic mice. This fragment coincides with the major area of hypersensitivity 'in vivo' and contains a number of DNasel footprints. Bandshift analysis shows that these footprints correspond to binding sites for the erythroid specific proteins GATA1 and NF-E2 and a number of ubiquitous proteins, including jun/fos, Sp1 and TEF2.

Entities: Disease Gene Species

Mesh：

Substances：

Year: 1991 PMID： 2027748 PMCID： PMC333894 DOI： 10.1093/nar/19.7.1413

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

33 in total

1. Transcriptional activation and DNA binding by the erythroid factor GF-1/NF-E1/Eryf 1.

Authors: D I Martin; S H Orkin
Journal: Genes Dev Date: 1990-11 Impact factor: 11.361

2. In vitro binding of several cell-specific and ubiquitous nuclear proteins to the GT-I motif of the SV40 enhancer.

Authors: J H Xiao; I Davidson; M Macchi; R Rosales; M Vigneron; A Staub; P Chambon
Journal: Genes Dev Date: 1987-10 Impact factor: 11.361

3. Two tissue-specific factors bind the erythroid promoter of the human porphobilinogen deaminase gene.

Authors: V Mignotte; L Wall; E deBoer; F Grosveld; P H Romeo
Journal: Nucleic Acids Res Date: 1989-01-11 Impact factor: 16.971

4. The human beta-globin gene 3' enhancer contains multiple binding sites for an erythroid-specific protein.

Authors: L Wall; E deBoer; F Grosveld
Journal: Genes Dev Date: 1988-09 Impact factor: 11.361

5. An erythrocyte-specific DNA-binding factor recognizes a regulatory sequence common to all chicken globin genes.

Authors: T Evans; M Reitman; G Felsenfeld
Journal: Proc Natl Acad Sci U S A Date: 1988-08 Impact factor: 11.205

6. Analysis of the tissue-specific enhancer at the 3' end of the chicken adult beta-globin gene.

Authors: B M Emerson; J M Nickol; P D Jackson; G Felsenfeld
Journal: Proc Natl Acad Sci U S A Date: 1987-07 Impact factor: 11.205

7. A dominant control region from the human beta-globin locus conferring integration site-independent gene expression.

Authors: D Talbot; P Collis; M Antoniou; M Vidal; F Grosveld; D R Greaves
Journal: Nature Date: 1989-03-23 Impact factor: 49.962

8. The beta-globin dominant control region activates homologous and heterologous promoters in a tissue-specific manner.

Authors: G Blom van Assendelft; O Hanscombe; F Grosveld; D R Greaves
Journal: Cell Date: 1989-03-24 Impact factor: 41.582

9. Gamma delta beta-thalassaemias 1 and 2 are the result of a 100 kbp deletion in the human beta-globin cluster.

Authors: R Taramelli; D Kioussis; E Vanin; K Bartram; J Groffen; J Hurst; F G Grosveld
Journal: Nucleic Acids Res Date: 1986-09-11 Impact factor: 16.971

10. Position-independent, high-level expression of the human beta-globin gene in transgenic mice.

Authors: F Grosveld; G B van Assendelft; D R Greaves; G Kollias
Journal: Cell Date: 1987-12-24 Impact factor: 41.582

45 in total

1. The polyoma virus enhancer cannot substitute for DNase I core hypersensitive sites 2-4 in the human beta-globin LCR.

Authors: K Tanimoto; Q Liu; J Bungert; J D Engel
Journal: Nucleic Acids Res Date: 1999-08-01 Impact factor: 16.971

2. Characterization of the factors binding to a PEPCK gene upstream hypersensitive site with LCR activity.

Authors: T E Cheyette; T Ip; S Faber; Y Matsui; R Chalkley
Journal: Nucleic Acids Res Date: 1992-07-11 Impact factor: 16.971

3. LCR/MEL: a versatile system for high-level expression of heterologous proteins in erythroid cells.

Authors: M Needham; C Gooding; K Hudson; M Antoniou; F Grosveld; M Hollis
Journal: Nucleic Acids Res Date: 1992-03-11 Impact factor: 16.971

4. Sequences within and flanking hypersensitive sites 3 and 2 of the beta-globin locus control region required for synergistic versus additive interaction with the epsilon-globin gene promoter.

Authors: J D Jackson; W Miller; R C Hardison
Journal: Nucleic Acids Res Date: 1996-11-01 Impact factor: 16.971

5. An Erythroid-Specific Chromatin Opening Element Increases β-Globin Gene Expression from Integrated Retroviral Gene Transfer Vectors.

Authors: Michael J Nemeth; Christopher H Lowrey
Journal: Gene Ther Mol Biol Date: 2004-12

6. Expression of GATA-1 in a non-hematopoietic cell line induces beta-globin locus control region chromatin structure remodeling and an erythroid pattern of gene expression.

Authors: Michael E Layon; Catherine J Ackley; Rachel J West; Christopher H Lowrey
Journal: J Mol Biol Date: 2006-12-06 Impact factor: 5.469

10. The locus control region is necessary for gene expression in the human beta-globin locus but not the maintenance of an open chromatin structure in erythroid cells.

Authors: A Reik; A Telling; G Zitnik; D Cimbora; E Epner; M Groudine
Journal: Mol Cell Biol Date: 1998-10 Impact factor: 4.272