Literature DB >> 2026704

Structural analysis of biologically active peptides and recombinant proteins and their modified counterparts by mass spectrometry.

B N Pramanik1, A Tsarbopoulos, J E Labdon, P P Trotta, T L Nagabhushan.   

Abstract

The structural characterization of the Escherichia coli-expressed human interferon alpha-2b (rh-IFN alpha-2b) was carried out by employing the fast atom bombardment (FAB) and plasma desorption (PD) mapping methods. The mass spectral data of the rh-IFN alpha-2b and the trypsin-generated peptide mixture allowed rapid and facile confirmation of the cDNA-derived sequence and determination of the existing disulfide pattern in the protein molecule. The same PD/FAB mapping approach was successfully employed in the structural determination of the iodination reaction product of rh-IFN alpha-2b and the potent vasoconstrictor peptide endothelin.

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Year:  1991        PMID： 2026704     DOI： 10.1016/0378-4347(91)80593-2

Source DB:  PubMed          Journal:  J Chromatogr


  4 in total

1.  Microwave-enhanced enzyme reaction for protein mapping by mass spectrometry: a new approach to protein digestion in minutes.

Authors:  Birendra N Pramanik; Urooj A Mirza; Yao Hain Ing; Yan-Hui Liu; Peter L Bartner; Patricia C Weber; Ajay K Bose
Journal:  Protein Sci       Date:  2002-11       Impact factor: 6.725

Review 2.  Fast atom bombardment mass spectrometric characterization of peptides.

Authors:  P R Das; B N Pramanik
Journal:  Mol Biotechnol       Date:  1998-04       Impact factor: 2.695

3.  Isolation and characterization of a monomethioninesulfoxide variant of interferon alpha-2b.

Authors:  G Gitlin; A Tsarbopoulos; S T Patel; W Sydor; B N Pramanik; S Jacobs; L Westreich; S Mittelman; J N Bausch
Journal:  Pharm Res       Date:  1996-05       Impact factor: 4.200

4.  Isolation and characterization of a resistant core peptide of recombinant human granulocyte-macrophage colony-stimulating factor (GM-CSF); confirmation of the GM-CSF amino acid sequence by mass spectrometry.

Authors:  A Tsarbopoulos; B N Pramanik; J E Labdon; P Reichert; G Gitlin; S Patel; V Sardana; T L Nagabhushan; P P Trotta
Journal:  Protein Sci       Date:  1993-11       Impact factor: 6.725
  4 in total

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