Literature DB >> 202572

A simple method for concentration of enteroviruses and rotaviruses from cell culture harvests using membrane filters.

S R Farrah, S M Goyal, C P Gerba, R H Conklin, C Wallis, J L Melnick, H L DuPont.   

Abstract

Organic compounds in cell culture harvests known as membrane-coating components (MCC) prevent virus adsorption to membrane filters. Blending cell culture harvests with fluorocarbon removed the MCC and permitted adsorption of virus in acidified harvests to epoxy-fiberglass filters. Subsequent elution with high pH buffer resulted in recovery of greater than 90% of the virus with concentrations of up to 100-fold.

Entities:  

Mesh：

Substances：

Year:  1978        PMID： 202572     DOI： 10.1159/000148921

Source DB:  PubMed          Journal:  Intervirology        ISSN： 0300-5526            Impact factor:   1.763


  11 in total

1.  Comparison between adsorption of poliovirus and rotavirus by aluminum hydroxide and activated sludge flocs.

Authors:  S R Farrah; S M Goyal; C P Gerba; R H Conklin; E M Smith
Journal:  Appl Environ Microbiol       Date:  1978-02       Impact factor: 4.792

2.  Methodology for enumeration of coliphages in foods.

Authors:  J E Kennedy; C I Wei; J L Oblinger
Journal:  Appl Environ Microbiol       Date:  1986-05       Impact factor: 4.792

3.  Simple method for concentration of bacteria from large volumes of tap water.

Authors:  S M Goyal; C P Gerba
Journal:  Appl Environ Microbiol       Date:  1980-11       Impact factor: 4.792

4.  Elution of poliovirus adsorbed to membrane filters.

Authors:  S R Farrah; G Bitton
Journal:  Appl Environ Microbiol       Date:  1978-12       Impact factor: 4.792

5.  Method for detecting viruses in aerosols.

Authors:  C Wallis; J L Melnick; V C Rao; T E Sox
Journal:  Appl Environ Microbiol       Date:  1985-11       Impact factor: 4.792

6.  Round robin investigation of methods for the recovery of poliovirus from drinking water.

Authors:  J L Melnick; R Safferman; V C Rao; S Goyal; G Berg; D R Dahling; B A Wright; E Akin; R Stetler; C Sorber
Journal:  Appl Environ Microbiol       Date:  1984-01       Impact factor: 4.792

7.  Rotavirus concentration from cell culture harvests: trypsin treatment followed by hydroextraction.

Authors:  S Ramia; S A Sattar
Journal:  Appl Environ Microbiol       Date:  1980-12       Impact factor: 4.792

8.  Development of a method for detection of human rotavirus in water and sewage.

Authors:  E M Smith; C P Gerba
Journal:  Appl Environ Microbiol       Date:  1982-06       Impact factor: 4.792

9.  Concentration of seeded simian rotavirus SA-11 from potable waters by using talc-celite layers and hydroextraction.

Authors:  S Ramia; S A Sattar
Journal:  Appl Environ Microbiol       Date:  1980-03       Impact factor: 4.792

10.  Simple method for the concentration of influenza virus from allantoic fluid on microporous filters.

Authors:  S M Goyal; H Hanssen; C P Gerba
Journal:  Appl Environ Microbiol       Date:  1980-03       Impact factor: 4.792
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.