Literature DB >> 2025244

Active recombinant C3a of human anaphylatoxin produced in Escherichia coli.

Y Fukuoka1, A Yasui, T Tachibana.   

Abstract

DNA sequence coding for the complete human C3a with 77 amino acids was divided into three portions, synthesized separately and constructed for expression in Escherichia coli. High expression of the recombinant C3a was achieved by an expression system using T7 polymerase. Purified recombinant C3a showed the same activities of ileum contraction and platelet aggregation of guinea pig as C3a purified from human serum.

Entities:  

Mesh：

Substances：

Year:  1991        PMID： 2025244     DOI： 10.1016/0006-291x(91)91683-4

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  4 in total

1.  New nucleotide sequence data on the EMBL File Server.

Authors: 
Journal:  Nucleic Acids Res       Date:  1991-10-11       Impact factor: 16.971

2.  Acylation-stimulating protein (ASP): structure-function determinants of cell surface binding and triacylglycerol synthetic activity.

Authors:  I Murray; J Köhl; K Cianflone
Journal:  Biochem J       Date:  1999-08-15       Impact factor: 3.857

3.  C3a is a chemotaxin for human eosinophils but not for neutrophils. I. C3a stimulation of neutrophils is secondary to eosinophil activation.

Authors:  P J Daffern; P H Pfeifer; J A Ember; T E Hugli
Journal:  J Exp Med       Date:  1995-06-01       Impact factor: 14.307

4.  Human C3a and C3a desArg anaphylatoxins have conserved structures, in contrast to C5a and C5a desArg.

Authors:  Goran Bajic; Laure Yatime; Andreas Klos; Gregers Rom Andersen
Journal:  Protein Sci       Date:  2012-12-16       Impact factor: 6.725
  4 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.