Literature DB >> 2024468

Analysis of the protein composition of alfalfa mosaic virus RNA-dependent RNA polymerase.

R Quadt1, H J Rosdorff, T W Hunt, E M Jaspars.   

Abstract

RNA-dependent RNA polymerase (RdRp) was solubilized and purified from cellular membranes isolated from alfalfa mosaic virus (AIMV)-infected tobacco by employing a procedure recently described for brome mosaic virus RdRp [R. Quadt and E.M.J. Jaspars, 1990, Virology 178, 189-194]. The purified AIMV RdRp is completely dependent on added template RNAs and exhibits a high degree of template specificity. Analysis of the protein composition of AIMV RdRp showed that AIMV-encoded proteins P1 and P2 and the coat protein (CP) are present in the active enzyme complex. Minus-strand synthesis by the AIMV RdRp is inhibited by AIMV CP. Native double-stranded AIMV RNAs are utilized as template for viral RNA synthesis by AIMV RdRp indicating that a helicase activity is present in the purified AIMV RdRp preparation.

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Year:  1991        PMID： 2024468     DOI： 10.1016/0042-6822(91)90674-z

Source DB:  PubMed          Journal:  Virology        ISSN： 0042-6822            Impact factor:   3.616


  31 in total

1.  The tobacco mosaic virus RNA polymerase complex contains a plant protein related to the RNA-binding subunit of yeast eIF-3.

Authors:  T A Osman; K W Buck
Journal:  J Virol       Date:  1997-08       Impact factor: 5.103

2.  A conserved hairpin structure in Alfamovirus and Bromovirus subgenomic promoters is required for efficient RNA synthesis in vitro.

Authors:  P C Haasnoot; F T Brederode; R C Olsthoorn; J F Bol
Journal:  RNA       Date:  2000-05       Impact factor: 4.942

3.  Spatial determinants of the alfalfa mosaic virus coat protein binding site.

Authors:  Siana M Laforest; Lee Gehrke
Journal:  RNA       Date:  2004-01       Impact factor: 4.942

4.  In vitro transcription by the turnip yellow mosaic virus RNA polymerase: a comparison with the alfalfa mosaic virus and brome mosaic virus replicases.

Authors:  B A Deiman; P W Verlaan; C W Pleij
Journal:  J Virol       Date:  2000-01       Impact factor: 5.103

5.  Mutations in coat protein binding sites of alfalfa mosaic virus RNA 3 affect subgenomic RNA 4 accumulation and encapsidation of viral RNAs.

Authors:  C B Reusken; L Neeleman; F T Brederode; J F Bol
Journal:  J Virol       Date:  1997-11       Impact factor: 5.103

6.  VP3, a structural protein of infectious pancreatic necrosis virus, interacts with RNA-dependent RNA polymerase VP1 and with double-stranded RNA.

Authors:  Torunn Pedersen; Astrid Skjesol; Jorunn B Jørgensen
Journal:  J Virol       Date:  2007-04-11       Impact factor: 5.103

Review 7.  A core promoter hairpin is essential for subgenomic RNA synthesis in alfalfa mosaic alfamovirus and is conserved in other Bromoviridae.

Authors:  E M Jaspars
Journal:  Virus Genes       Date:  1998       Impact factor: 2.332

8.  Role of an essential triloop hairpin and flanking structures in the 3' untranslated region of Alfalfa mosaic virus RNA in in vitro transcription.

Authors:  René C L Olsthoorn; John F Bol
Journal:  J Virol       Date:  2002-09       Impact factor: 5.103

9.  Purification of the cucumber necrosis virus replicase from yeast cells: role of coexpressed viral RNA in stimulation of replicase activity.

Authors:  Zivile Panaviene; Tadas Panavas; Saulius Serva; Peter D Nagy
Journal:  J Virol       Date:  2004-08       Impact factor: 5.103

10.  The 3'-untranslated region of alfalfa mosaic virus RNA 3 contains at least two independent binding sites for viral coat protein.

Authors:  C B Reusken; L Neeleman; J F Bol
Journal:  Nucleic Acids Res       Date:  1994-04-25       Impact factor: 16.971
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