Literature DB >> 20238257

Protoplast transformation of filamentous fungi.

B Gillian Turgeon1, Bradford Condon, Jinyuan Liu, Ning Zhang.   

Abstract

The protoplast method for the transformation of filamentous fungi is described in detail, as is the Restriction Enzyme-Mediated Integration (REMI) procedure for introducing tagged mutations into the fungal genome. A split marker method for generating PCR fragments for targeted integration and deletion of genes of interest is also detailed.

Mesh：

Substances：

Year:  2010        PMID： 20238257     DOI： 10.1007/978-1-60761-611-5_1

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  15 in total

1.  Histidine kinase two-component response regulator proteins regulate reproductive development, virulence, and stress responses of the fungal cereal pathogens Cochliobolus heterostrophus and Gibberella zeae.

Authors:  Shinichi Oide; Jinyuan Liu; Sung-Hwan Yun; Dongliang Wu; Alex Michev; May Yee Choi; Benjamin A Horwitz; B Gillian Turgeon
Journal:  Eukaryot Cell       Date:  2010-10-29

2.  Structure-guided function discovery of an NRPS-like glycine betaine reductase for choline biosynthesis in fungi.

Authors:  Yang Hai; Arthur M Huang; Yi Tang
Journal:  Proc Natl Acad Sci U S A       Date:  2019-05-06       Impact factor: 11.205

3.  Development of a flow cytometry-based plating-free system for strain engineering in industrial fungi.

Authors:  Yu-Jing Yang; Yin Liu; Dan-Dan Liu; Wen-Zhu Guo; Li-Xian Wang; Xing-Ji Wang; He-Xin Lv; Yang Yang; Qian Liu; Chao-Guang Tian
Journal:  Appl Microbiol Biotechnol       Date:  2021-12-18       Impact factor: 4.813

4.  Complementary symbiont contributions to plant decomposition in a fungus-farming termite.

Authors:  Michael Poulsen; Haofu Hu; Cai Li; Zhensheng Chen; Luohao Xu; Saria Otani; Sanne Nygaard; Tania Nobre; Sylvia Klaubauf; Philipp M Schindler; Frank Hauser; Hailin Pan; Zhikai Yang; Anton S M Sonnenberg; Z Wilhelm de Beer; Yong Zhang; Michael J Wingfield; Cornelis J P Grimmelikhuijzen; Ronald P de Vries; Judith Korb; Duur K Aanen; Jun Wang; Jacobus J Boomsma; Guojie Zhang
Journal:  Proc Natl Acad Sci U S A       Date:  2014-09-22       Impact factor: 11.205

5.  Gene Disruption in Scedosporium aurantiacum: Proof of Concept with the Disruption of SODC Gene Encoding a Cytosolic Cu,Zn-Superoxide Dismutase.

Authors:  Victoire Pateau; Bienvenue Razafimandimby; Patrick Vandeputte; Christopher R Thornton; Thomas Guillemette; Jean-Philippe Bouchara; Sandrine Giraud
Journal:  Mycopathologia       Date:  2017-10-11       Impact factor: 2.574

6.  ChLae1 and ChVel1 regulate T-toxin production, virulence, oxidative stress response, and development of the maize pathogen Cochliobolus heterostrophus.

Authors:  Dongliang Wu; Shinichi Oide; Ning Zhang; May Yee Choi; B Gillian Turgeon
Journal:  PLoS Pathog       Date:  2012-02-23       Impact factor: 6.823

7.  Sm2, a paralog of the Trichoderma cerato-platanin elicitor Sm1, is also highly important for plant protection conferred by the fungal-root interaction of Trichoderma with maize.

Authors:  Romana Gaderer; Netta L Lamdan; Alexa Frischmann; Michael Sulyok; Rudolf Krska; Benjamin A Horwitz; Verena Seidl-Seiboth
Journal:  BMC Microbiol       Date:  2015-01-16       Impact factor: 3.605

8.  Comparative Genomic Analysis of Drechmeria coniospora Reveals Core and Specific Genetic Requirements for Fungal Endoparasitism of Nematodes.

Authors:  Kevin Lebrigand; Le D He; Nishant Thakur; Marie-Jeanne Arguel; Jolanta Polanowska; Bernard Henrissat; Eric Record; Ghislaine Magdelenat; Valérie Barbe; Sylvain Raffaele; Pascal Barbry; Jonathan J Ewbank
Journal:  PLoS Genet       Date:  2016-05-06       Impact factor: 5.917

9.  Mating-type genes of the anamorphic fungus Ulocladium botrytis affect both asexual sporulation and sexual reproduction.

Authors:  Qun Wang; Shi Wang; Chen Lin Xiong; Timothy Y James; Xiu Guo Zhang
Journal:  Sci Rep       Date:  2017-08-11       Impact factor: 4.379

10.  PacC and pH-dependent transcriptome of the mycotrophic fungus Trichoderma virens.

Authors:  Naomi Trushina; Michal Levin; Prasun K Mukherjee; Benjamin A Horwitz
Journal:  BMC Genomics       Date:  2013-02-28       Impact factor: 3.969
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