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Literature DB >> 202375

Methods for the study of the glycoproteins and proteoglycans of bone using bacterial collagenase. Determination of bone sialoprotein and chondroitin sulphate.

Abstract

A method has been developed for the solubilization of bone matrix using bacterial collagenase. The soluble macromolecules were separated by a column procedure on DEAE-cellulose and analysed for uronic and sialic acids. Values for the total non-collagenous fraction of bone and its content of bone sialoprotein and chondroitin sulphate were obtained. The method was compared with similar procedures using EDTA extraction and papain digestion.

Entities: Chemical

Mesh：

Substances：

Year: 1977 PMID： 202375 DOI： 10.1007/bf02223293

Source DB: PubMed Journal: Calcif Tissue Res ISSN： 0008-0594

35 in total

1. Components of the organic matrices of bone and dentine isolated only after digestion with collagenase.

Authors: A G Leaver; I B Holbrook; I L Jones; M Thomas; L Sheil
Journal: Arch Oral Biol Date: 1975-03 Impact factor: 2.633

2. The binding of hydrogen ions to an acidic glycoprotein from bovine cortical bone.

Authors: A R Peacocke; P A Williams
Journal: Biochem J Date: 1967-12 Impact factor: 3.857

3. On the presence of sialic acid in certain glycoproteins and in gangliosides.

Authors: I WERNER; L ODIN
Journal: Acta Soc Med Ups Date: 1952

4. Studies on bone matrix glycoproteins. Incorporation of (1-14C)glucosamine and plasma (14C)glycoprotein into rabbit cortical bone.

Authors: J T Triffitt; M Owen
Journal: Biochem J Date: 1973-09 Impact factor: 3.857

5. Use of a mixture of proteinase-free collagenases for the specific assay of radioactive collagen in the presence of other proteins.

Authors: B Peterkofsky; R Diegelmann
Journal: Biochemistry Date: 1971-03-16 Impact factor: 3.162

6. A method for the study of bone mucosubstances by using collagenase.

Authors: D Oldroyd; G M Herring
Journal: Biochem J Date: 1967-08 Impact factor: 3.857

7. Studies on the peptides, free amino acids and certain related compounds isolated from ox bone.

Authors: A G Leaver; C A Shuttleworth
Journal: Arch Oral Biol Date: 1968-05 Impact factor: 2.633

8. The neutral lipids of bovine bone.

Authors: I M Shapiro
Journal: Arch Oral Biol Date: 1971-04 Impact factor: 2.633

9. The physical properties of a glycoprotein from bovine cortical bone (bone sialoprotein).

Authors: P A Williams; A R Peacocke
Journal: Biochim Biophys Acta Date: 1965-11-01

10. DETERMINATION OF GLYCOSAMINOGLYCANS (MUCOPOLYSACCHARIDES) FROM TISSUE ON THE MICROGRAM SCALE.

Authors: C A ANTONOPOULOS; S GARDELL; J A SZIRMAI; E R DETYSSONSK
Journal: Biochim Biophys Acta Date: 1964-03-02

6 in total

1. Solubilized and insolubilized bone morphogenetic protein.

Authors: M R Urist; A Mikulski; A Lietze
Journal: Proc Natl Acad Sci U S A Date: 1979-04 Impact factor: 11.205

2. Analysis of the heterogeneity of human collagens by two-dimensional polyacrylamide-gel electrophoresis.

Authors: W G Cole; D Chan
Journal: Biochem J Date: 1981-08-01 Impact factor: 3.857

3. Chemical composition of normal and osteoarthrotic cancellous bone of the femoral head. Studies of EDTA extracts and collagenase digests.

Authors: J M Mbuyi-Muamba; J Dequeker
Journal: Arch Orthop Trauma Surg Date: 1984