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Literature DB >> 20234915

In situ azide formation and "click" reaction of nile red with DNA as an alternative postsynthetic route.

Christoph Beyer¹, Hans-Achim Wagenknecht.

Abstract

Oligonucleotides that contain a 5-iodo-2'-deoxyuridine in the sequence can be applied for a postsynthetic "click"-type modification with ethynyl-modified nile red as a fluorescent label by in situ formation of the intermediate azide.

Entities: Chemical

Mesh：

Substances：

Year: 2010 PMID： 20234915 DOI： 10.1039/b924471a

Source DB: PubMed Journal: Chem Commun (Camb) ISSN： 1359-7345 Impact factor: 6.222

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Cited

6 in total

1. Strain Promoted Click Chemistry of 2- or 8-Azidopurine and 5-Azidopyrimidine Nucleosides and 8-Azidoadenosine Triphosphate with Cyclooctynes. Application to Living Cell Fluorescent Imaging.

Authors: Jessica Zayas; Marie Annoual; Jayanta Kumar Das; Quentin Felty; Walter G Gonzalez; Jaroslava Miksovska; Nima Sharifai; Akira Chiba; Stanislaw F Wnuk
Journal: Bioconjug Chem Date: 2015-07-02 Impact factor: 4.774

2. Enzymatic incorporation of an azide-modified UTP analog into oligoribonucleotides for post-transcriptional chemical functionalization.

Authors: Harita Rao; Arun A Tanpure; Anupam A Sawant; Seergazhi G Srivatsan
Journal: Nat Protoc Date: 2012-05-10 Impact factor: 13.491

Review 6. Chemical architecture and applications of nucleic acid derivatives containing 1,2,3-triazole functionalities synthesized via click chemistry.

Authors: Tim Efthymiou; Wei Gong; Jean-Paul Desaulniers
Journal: Molecules Date: 2012-10-26 Impact factor: 4.411

6 in total

In situ azide formation and "click" reaction of nile red with DNA as an alternative postsynthetic route.

1. Strain Promoted Click Chemistry of 2- or 8-Azidopurine and 5-Azidopyrimidine Nucleosides and 8-Azidoadenosine Triphosphate with Cyclooctynes. Application to Living Cell Fluorescent Imaging.

2. Enzymatic incorporation of an azide-modified UTP analog into oligoribonucleotides for post-transcriptional chemical functionalization.

3. DNA modification under mild conditions by Suzuki-Miyaura cross-coupling for the generation of functional probes.

4. Synthesis and optical properties of pyrrolidinyl peptide nucleic acid carrying a clicked Nile red label.

5. A versatile toolbox for posttranscriptional chemical labeling and imaging of RNA.

Review 6. Chemical architecture and applications of nucleic acid derivatives containing 1,2,3-triazole functionalities synthesized via click chemistry.