H Nishi1, K Ohta, M Takechi, S Yoneda, M Hiraoka, N Kamata. 1. Department of Oral and Maxillofacial Surgery, Division of Cervico-Gnathostmatology, Programs for Applied Biomedicine, Graduate School of Biomedical Sciences, Hiroshima University, Minami-Ku, Hiroshima, Japan. hiyoko@hiroshima-u.ac.jp
Abstract
OBJECTIVE: The purpose of this study was to develop a graft material made of gingival fibroblasts cultured in animal-free medium (HFDM1). METHODS: We examined the effects of human serum (HS) on cell growth and wound healing capability, demonstrated by cytokine production, of gingival fibroblasts cultured in HFDM1. Subsequently, the capability of fibroblasts cultured in HFDM1 with 2% HS to promote the healing of skin defects was evaluated using nude mice. RESULTS: The proliferation of human gingival fibroblasts was increased when HS at a concentration of 0.5-2% was added to HFDM1. Wound healing cytokines, including transforming growth factor-beta, keratinocyte growth factor, hepatocyte growth factor, vascular endothelial growth factor, and IL-6 produced by gingival fibroblasts were increased by adding 2% HS to HFDM1. In addition, gingival fibroblasts cultured in HFDM1 with 2% HS improved wound healing of mouse skin defects as well as those cultured in Dulbecco's modified Eagle's medium with 10% fetal calf serum. CONCLUSION: Gingival fibroblasts cultured in HFDM1 with 2% HS may be useful as a graft material for reconstruction.
OBJECTIVE: The purpose of this study was to develop a graft material made of gingival fibroblasts cultured in animal-free medium (HFDM1). METHODS: We examined the effects of human serum (HS) on cell growth and wound healing capability, demonstrated by cytokine production, of gingival fibroblasts cultured in HFDM1. Subsequently, the capability of fibroblasts cultured in HFDM1 with 2% HS to promote the healing of skin defects was evaluated using nude mice. RESULTS: The proliferation of human gingival fibroblasts was increased when HS at a concentration of 0.5-2% was added to HFDM1. Wound healing cytokines, including transforming growth factor-beta, keratinocyte growth factor, hepatocyte growth factor, vascular endothelial growth factor, and IL-6 produced by gingival fibroblasts were increased by adding 2% HS to HFDM1. In addition, gingival fibroblasts cultured in HFDM1 with 2% HS improved wound healing of mouseskin defects as well as those cultured in Dulbecco's modified Eagle's medium with 10% fetal calf serum. CONCLUSION: Gingival fibroblasts cultured in HFDM1 with 2% HS may be useful as a graft material for reconstruction.
Authors: Christine Linard; Frederique Tissedre; Elodie Busson; Valerie Holler; Thomas Leclerc; Carine Strup-Perrot; Ludovic Couty; Bruno L'homme; Marc Benderitter; Antoine Lafont; Jean Jacques Lataillade; Bernard Coulomb Journal: Stem Cells Dev Date: 2015-02-26 Impact factor: 3.272
Authors: Rana Tarzemany; Guoqiao Jiang; Jean X Jiang; Corrie Gallant-Behm; Colin Wiebe; David A Hart; Hannu Larjava; Lari Häkkinen Journal: Exp Cell Res Date: 2018-03-27 Impact factor: 3.905