Literature DB >> 20230890

Homogenous, real-time duplex loop-mediated isothermal amplification using a single fluorophore-labeled primer and an intercalator dye: Its application to the simultaneous detection of Shiga toxin genes 1 and 2 in Shiga toxigenic Escherichia coli isolates.

Yoshihiro Kouguchi1, Takako Fujiwara, Miki Teramoto, Mika Kuramoto.   

Abstract

We developed a completely homogeneous duplex loop-mediated isothermal amplification (LAMP) method. The present LAMP method employed a combination of a 6-carboxyfluorescein (FAM)-labeled primer (donor) for one target gene, a non-labeled primer for the other, and an intercalator ethidium bromide (EtBr) dye (acceptor) on the basis of fluorescence resonance energy transfer (FRET) between the FAM donor and EtBr acceptor. Measuring changes in fluorescence of FAM enabled the LAMP method to detect two different genes simultaneously. This method was used to detect Shiga toxin genes in Shiga toxigenic Escherichia coli isolates, demonstrating simultaneous detection of two different genes with rapidity and accuracy. Copyright 2010 Elsevier Ltd. All rights reserved.

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Year:  2010        PMID： 20230890     DOI： 10.1016/j.mcp.2010.03.001

Source DB:  PubMed          Journal:  Mol Cell Probes        ISSN： 0890-8508            Impact factor:   2.365


  11 in total

1.  Evaluation of a loop-mediated isothermal amplification suite for the rapid, reliable, and robust detection of Shiga toxin-producing Escherichia coli in produce.

Authors:  Fei Wang; Qianru Yang; Yinzhi Qu; Jianghong Meng; Beilei Ge
Journal:  Appl Environ Microbiol       Date:  2014-02-07       Impact factor: 4.792

2.  Real-time kinetics and high-resolution melt curves in single-molecule digital LAMP to differentiate and study specific and non-specific amplification.

Authors:  Justin C Rolando; Erik Jue; Jacob T Barlow; Rustem F Ismagilov
Journal:  Nucleic Acids Res       Date:  2020-04-17       Impact factor: 16.971

3.  Loop-mediated isothermal amplification assays for detecting shiga toxin-producing Escherichia coli in ground beef and human stools.

Authors:  Fei Wang; Lin Jiang; Beilei Ge
Journal:  J Clin Microbiol       Date:  2011-10-26       Impact factor: 5.948

4.  Rapid detection of Streptococcus pneumoniae by real-time fluorescence loop-mediated isothermal amplification.

Authors:  Yong Xia; Xu-Guang Guo; Shan Zhou
Journal:  J Thorac Dis       Date:  2014-09       Impact factor: 2.895

5.  Rapid and specific detection of escherichia coli serogroups O26, O45, O103, O111, O121, O145, and O157 in ground beef, beef trim, and produce by loop-mediated isothermal amplification.

Authors:  Fei Wang; Lin Jiang; Qianru Yang; Witoon Prinyawiwatkul; Beilei Ge
Journal:  Appl Environ Microbiol       Date:  2012-02-10       Impact factor: 4.792

6.  Development of double loop-mediated isothermal amplification to detect Listeria monocytogenes in food.

Authors:  Rina Wu; Xiang Liu; Bangcheng Guo; Fusheng Chen; Xiaohong Wang
Journal:  Curr Microbiol       Date:  2014-08-03       Impact factor: 2.188

7.  Development of a multiplex loop-mediated isothermal amplification assay to detect shiga toxin-producing Escherichia coli in cattle.

Authors:  Hee-Jin Dong; Ae-Ri Cho; Tae-Wook Hahn; Seongbeom Cho
Journal:  J Vet Sci       Date:  2014-03-21       Impact factor: 1.672

8.  Real-time Detection and Monitoring of Loop Mediated Amplification (LAMP) Reaction Using Self-quenching and De-quenching Fluorogenic Probes.

Authors:  Vijay J Gadkar; David M Goldfarb; Soren Gantt; Peter A G Tilley
Journal:  Sci Rep       Date:  2018-04-03       Impact factor: 4.379

9.  Establishment of a universal and rational gene detection strategy through three-way junction-based remote transduction.

Authors:  Yidan Tang; Baiyang Lu; Zhentong Zhu; Bingling Li
Journal:  Chem Sci       Date:  2017-11-27       Impact factor: 9.825

10.  Rapid, simple and direct detection of Meloidogyne hapla from infected root galls using loop-mediated isothermal amplification combined with FTA technology.

Authors:  Huan Peng; Haibo Long; Wenkun Huang; Jing Liu; Jiangkuan Cui; Lingan Kong; Xianqi Hu; Jianfeng Gu; Deliang Peng
Journal:  Sci Rep       Date:  2017-04-03       Impact factor: 4.379
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