| Literature DB >> 20230772 |
Wei-Tao Cong1, Zhong-Xin Zhu, Hong-Zhang He, Yan Jin, Cheng-Xi Jiang, Jung-Kap Choi, Li-Tai Jin, Xiao-Kun Li.
Abstract
We describe a visible dye-based staining method for DNA in polyacrylamide gels using ethyl violet (EV). The novel method is a background-free, sensitive, economical, and simple procedure involving only staining and washing steps that can be completed within 30 min. As little as 0.8-1.6 ng of phiX174 DNA/HaeIII can be detected by EV, which is about eightfold more sensitive than Nile blue (NB) stain and twofold less sensitive than ethidium bromide (EB) stain. 2010 Elsevier Inc. All rights reserved.Entities:
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Year: 2010 PMID： 20230772 DOI： 10.1016/j.ab.2010.03.017
Source DB: PubMed Journal: Anal Biochem ISSN： 0003-2697 Impact factor: 3.365