BACKGROUND: Human bocavirus (HBoV) is a widespread human parvovirus causing acute respiratory illness in young children. The HBoV primary infections are viremic and can be diagnosed serologically. OBJECTIVES: To set up HBoV-IgG-avidity enzyme immuno assays (EIAs) using as antigen recombinant VP2 virus-like particles (VLPs), for diagnosis and timing of primary infections and their distinction from secondary infections or immunoactivations by this recently found virus. STUDY DESIGN: The VLPs were utilized in setting up HBoV-IgG-avidity-EIAs of two different types. Paired sera were available from 36 wheezing children with acute primary HBoV infection, single sera from 108 nonsymptomatic university students, and 84 single or follow-up sera from 38 adults with pre-existing HBoV immunity. RESULTS: HBoV-IgG avidity for the VP2-VLPs was measured successfully by protein-denaturing EIAs of two types, employing low concentrations of urea (4.7M and 2.5M). The diagnostic specificities were 99.1% and 90.7%, and diagnostic sensitivities, 94.4% and 91.7%, respectively. Interestingly, of the adults followed up 44% (4/9) exhibited significant titre increases of past-immunity HBoV-IgG. CONCLUSIONS: Diagnosis of HBoV primary infection can be strengthened by measurement of IgG avidity. HBoV secondary infections or anamnestic antibody responses occur ubiquitously in immunocompetent adults. Copyright 2010 Elsevier B.V. All rights reserved.
BACKGROUND:Human bocavirus (HBoV) is a widespread human parvovirus causing acute respiratory illness in young children. The HBoV primary infections are viremic and can be diagnosed serologically. OBJECTIVES: To set up HBoV-IgG-avidity enzyme immuno assays (EIAs) using as antigen recombinant VP2 virus-like particles (VLPs), for diagnosis and timing of primary infections and their distinction from secondary infections or immunoactivations by this recently found virus. STUDY DESIGN: The VLPs were utilized in setting up HBoV-IgG-avidity-EIAs of two different types. Paired sera were available from 36 wheezingchildren with acute primary HBoV infection, single sera from 108 nonsymptomatic university students, and 84 single or follow-up sera from 38 adults with pre-existing HBoV immunity. RESULTS:HBoV-IgG avidity for the VP2-VLPs was measured successfully by protein-denaturing EIAs of two types, employing low concentrations of urea (4.7M and 2.5M). The diagnostic specificities were 99.1% and 90.7%, and diagnostic sensitivities, 94.4% and 91.7%, respectively. Interestingly, of the adults followed up 44% (4/9) exhibited significant titre increases of past-immunity HBoV-IgG. CONCLUSIONS: Diagnosis of HBoV primary infection can be strengthened by measurement of IgG avidity. HBoV secondary infections or anamnestic antibody responses occur ubiquitously in immunocompetent adults. Copyright 2010 Elsevier B.V. All rights reserved.
Authors: Kalle Kantola; Lea Hedman; Jane Arthur; Abdiwahab Alibeto; Eric Delwart; Tuomas Jartti; Olli Ruuskanen; Klaus Hedman; Maria Söderlund-Venermo Journal: J Infect Dis Date: 2011-09-15 Impact factor: 5.226
Authors: Anna Grahn; Marie Studahl; Staffan Nilsson; Elisabeth Thomsson; Malin Bäckström; Tomas Bergström Journal: Clin Vaccine Immunol Date: 2011-06-22
Authors: Colin P Sharp; Matthew LeBreton; Kalle Kantola; Ahmadou Nana; Joseph Le Doux Diffo; Cyrille F Djoko; Ubald Tamoufe; John A Kiyang; Tafon G Babila; Eitel Mpoudi Ngole; Oliver G Pybus; Eric Delwart; Eric Delaporte; Martine Peeters; Maria Soderlund-Venermo; Klaus Hedman; Nathan D Wolfe; Peter Simmonds Journal: J Virol Date: 2010-07-28 Impact factor: 5.103
Authors: Tingting Chen; Lea Hedman; Petri S Mattila; Laura Jartti; Tuomas Jartti; Olli Ruuskanen; Maria Söderlund-Venermo; Klaus Hedman Journal: PLoS One Date: 2012-08-03 Impact factor: 3.240