| Literature DB >> 20226324 |
Kyoung Ho Roh1, Sinyoung Kim, Chang-Ki Kim, Jong Hwa Yum, Myung Sook Kim, Dongeun Yong, Seok Hoon Jeong, Kyungwon Lee, June Myung Kim, Yunsop Chong.
Abstract
Of 276 nonduplicate Bacteroides fragilis clinical isolates recovered from 1997 to 2004, 3 were resistant to carbapenem. cepA and cfiA alleles were detected by polymerase chain reaction in 240 (87.0%) and 11 (4.0%) of the isolates, respectively. Insertion sequence (IS) elements were found only in the 3 carbapenem-resistant B. fragilis isolates, which produced metallo-beta-lactamase at a level detectable by UV spectrophotometry. Sequence analysis showed 1 new cfiA variant, cfiA(11), and 2 novel IS elements. The cfiA(11) gene revealed 5 amino acid substitutions compared to cfiA, with 97.6% amino acid identity. The transposase, terminal inverted repeat sequence, and target site duplication sequence of the 2 novel IS elements were unique. This study reconfirmed the correlation between ISs and carbapenem resistance in B. fragilis. 2010 Elsevier Inc. All rights reserved.Entities:
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Year: 2010 PMID： 20226324 DOI： 10.1016/j.diagmicrobio.2009.11.003
Source DB: PubMed Journal: Diagn Microbiol Infect Dis ISSN： 0732-8893 Impact factor: 2.803