| Literature DB >> 20224175 |
Hidetoshi Hasuwa1, Yuko Muro, Masahito Ikawa, Noriko Kato, Yoshihide Tsujimoto, Masaru Okabe.
Abstract
In the present paper, we introduce a transgenic mouse line whose sperm express green fluorescent protein (GFP) in their acrosome and red fluorescent protein (RFP) in their mitochondria [B6D2F1- Tg(CAG/su9-DsRed2, Acr3-EGFP)RBGS002Osb]. The dual fluorescent sperm showed normal fertilizing ability in both in vivo and in vitro fertilization and the sperm could be observed through uterine and oviductal walls when female reproductive tracts were dissected out and placed under excitation light. This characteristic could facilitate examination of sperm migration inside the female reproductive tract as well as facilitating in situ live imaging of the acrosome reaction, the details of which have remained elusive.Entities:
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Year: 2010 PMID： 20224175 DOI： 10.1538/expanim.59.105
Source DB: PubMed Journal: Exp Anim ISSN： 0007-5124