OBJECTIVE: Immature mammalian oocytes cultured in vitro undergo inadequate cytoplasmic maturation and hence have a limited potential for fertilization. Our primary objective was to determine if the addition of epidermal growth factor (EGF) to the in vitro culture system would have a positive effect on oocyte cytoplasmic maturation. DESIGN: We studied the effect of different EGF concentrations on both denuded and cumulus-enclosed mouse oocytes cultured in vitro. MAIN OUTCOME MEASURES: The percentage of oocytes undergoing germinal vesicle breakdown (GVBD) and polar body one formation over time as a function of EGF concentration was determined. RESULTS: A dose-related positive effect of EGF on both GVBD and polar body one formation over time was observed for mouse oocytes. As well, a similar effect of EGF was seen on immature human oocytes that had not been stimulated with exogenous gonadotropins. CONCLUSIONS: The use of EGF may allow for the performance of successful in vitro fertilization procedures using immature human oocytes retrieved during unstimulated cycles.
OBJECTIVE: Immature mammalian oocytes cultured in vitro undergo inadequate cytoplasmic maturation and hence have a limited potential for fertilization. Our primary objective was to determine if the addition of epidermal growth factor (EGF) to the in vitro culture system would have a positive effect on oocyte cytoplasmic maturation. DESIGN: We studied the effect of different EGF concentrations on both denuded and cumulus-enclosed mouse oocytes cultured in vitro. MAIN OUTCOME MEASURES: The percentage of oocytes undergoing germinal vesicle breakdown (GVBD) and polar body one formation over time as a function of EGF concentration was determined. RESULTS: A dose-related positive effect of EGF on both GVBD and polar body one formation over time was observed for mouse oocytes. As well, a similar effect of EGF was seen on immature human oocytes that had not been stimulated with exogenous gonadotropins. CONCLUSIONS: The use of EGF may allow for the performance of successful in vitro fertilization procedures using immature human oocytes retrieved during unstimulated cycles.
Authors: O M Avrech; G A Goldman; O Rufas; A Stein; S Amit; I Yoles; H Pinkas; B Fisch Journal: J Assist Reprod Genet Date: 1997-07 Impact factor: 3.412
Authors: D G Hammitt; C H Syrop; B J Van Voorhis; D L Walker; T M Miller; K M Barud; C C Hood Journal: J Assist Reprod Genet Date: 1992-10 Impact factor: 3.412
Authors: Kazuhiro Kawamura; Nanami Kawamura; Sabine M Mulders; Maarten D Sollewijn Gelpke; Aaron J W Hsueh Journal: Proc Natl Acad Sci U S A Date: 2005-06-20 Impact factor: 11.205
Authors: B Asimakopoulos; B Schöpper; A Dawson; G S Caglar; I Vakalopoulos; S Al-Hasani; K Diedrich; N Nikolettos Journal: J Endocrinol Invest Date: 2006-05 Impact factor: 4.256