BACKGROUND: A mutant Chlamydia trachomatis variant (nvC trachomatis) has made it more difficult to diagnose chlamydia in Sweden. The proportion of nvC trachomatis has varied between Swedish counties (25-80 %) in the period 2006-07. Our goal has been to monitor nvC trachomatis among our patients from January 2007 and up to July 2008. MATERIAL AND METHODS: In this time period, all C trachomatis samples at Fürst Medical Laboratory, Norway were analyzed twice. Cobas TaqMan 48 (Roche Diagnostics) was used to detect C trachomatis in isolated DNA and real-time PCR methods developed by us were used to both detect and verify nvC trachomatis. RESULTS: 61 patients of 23 726 patients were identified as carriers of nvC trachomatis. The proportion of C trachomatis carriers who were positive for nvC trachomatis increased from 1.0 % in the first quarter of 2007 to 3.2 % in the second quarter of 2008. INTERPRETATION: Our results show a slow but steady increase in the proportion of nvC trachomatis positive tests. As compared to previous rates reported in Sweden (25-80 %), the occurrence of nvC trachomatis in our data is low. The epidemiology of this chlamydia mutant contributes to the understanding of mechanisms for spread of sexually transmitted infections and emphasize that you only find what you are looking for.
BACKGROUND: A mutant Chlamydia trachomatis variant (nvC trachomatis) has made it more difficult to diagnose chlamydia in Sweden. The proportion of nvC trachomatis has varied between Swedish counties (25-80 %) in the period 2006-07. Our goal has been to monitor nvC trachomatis among our patients from January 2007 and up to July 2008. MATERIAL AND METHODS: In this time period, all C trachomatis samples at Fürst Medical Laboratory, Norway were analyzed twice. Cobas TaqMan 48 (Roche Diagnostics) was used to detect C trachomatis in isolated DNA and real-time PCR methods developed by us were used to both detect and verify nvC trachomatis. RESULTS: 61 patients of 23 726 patients were identified as carriers of nvC trachomatis. The proportion of C trachomatis carriers who were positive for nvC trachomatis increased from 1.0 % in the first quarter of 2007 to 3.2 % in the second quarter of 2008. INTERPRETATION: Our results show a slow but steady increase in the proportion of nvC trachomatis positive tests. As compared to previous rates reported in Sweden (25-80 %), the occurrence of nvC trachomatis in our data is low. The epidemiology of this chlamydia mutant contributes to the understanding of mechanisms for spread of sexually transmitted infections and emphasize that you only find what you are looking for.